TY - JOUR
T1 - Clonal characterization of the human IgG antibody repertoire to Haemophilus influenzae type b polysaccharide. II. IgG antibodies contain V(H) genes from a single V(H) family and V(L) genes from at least four V(L) families
AU - Scott, M. G.
AU - Tarrand, J. J.
AU - Crimmins, D. L.
AU - McCourt, D. W.
AU - Siegel, N. R.
AU - Smith, C. E.
AU - Nahm, M. H.
PY - 1989
Y1 - 1989
N2 - To define the V gene family repertoire of human IgG anti-Haemophilus influenzae type b polysaccharide antibodies, we purified six IgG1 and nine IgG2 anti-Hib-PS antibodies to monoclonality from immune serum of six individuals and performed N-terminal amino acid sequence analysis. Of the 15 clonal antibodies we examined, all H chain V regions were of the V(H)III family. In contrast, the L chains of these antibodies were clearly from at least four different V(L) families; V(K)I, V(K)II, V(K)III, and V(λ). Interestingly, V(L) family expression correlated with the cross-reactivity of these antibodies to the capsular carbohydrate of Escherichia coli K100. V(K)II antibodies did not cross-react, whereas antibodies expressing V(λ), V(K)I, or V(K)III generally cross-reacted. We conclude that L chain V regions are very important contributors to the limited heterogeneity in this antibody repertoire.
AB - To define the V gene family repertoire of human IgG anti-Haemophilus influenzae type b polysaccharide antibodies, we purified six IgG1 and nine IgG2 anti-Hib-PS antibodies to monoclonality from immune serum of six individuals and performed N-terminal amino acid sequence analysis. Of the 15 clonal antibodies we examined, all H chain V regions were of the V(H)III family. In contrast, the L chains of these antibodies were clearly from at least four different V(L) families; V(K)I, V(K)II, V(K)III, and V(λ). Interestingly, V(L) family expression correlated with the cross-reactivity of these antibodies to the capsular carbohydrate of Escherichia coli K100. V(K)II antibodies did not cross-react, whereas antibodies expressing V(λ), V(K)I, or V(K)III generally cross-reacted. We conclude that L chain V regions are very important contributors to the limited heterogeneity in this antibody repertoire.
UR - http://www.scopus.com/inward/record.url?scp=0024365916&partnerID=8YFLogxK
M3 - Article
C2 - 2499631
AN - SCOPUS:0024365916
SN - 0022-1767
VL - 143
SP - 293
EP - 298
JO - Journal of Immunology
JF - Journal of Immunology
IS - 1
ER -