Clonal characterization of the human IgG antibody repertoire to Haemophilus influenzae type b polysaccharide. II. IgG antibodies contain V(H) genes from a single V(H) family and V(L) genes from at least four V(L) families

M. G. Scott, J. J. Tarrand, D. L. Crimmins, D. W. McCourt, N. R. Siegel, C. E. Smith, M. H. Nahm

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Abstract

To define the V gene family repertoire of human IgG anti-Haemophilus influenzae type b polysaccharide antibodies, we purified six IgG1 and nine IgG2 anti-Hib-PS antibodies to monoclonality from immune serum of six individuals and performed N-terminal amino acid sequence analysis. Of the 15 clonal antibodies we examined, all H chain V regions were of the V(H)III family. In contrast, the L chains of these antibodies were clearly from at least four different V(L) families; V(K)I, V(K)II, V(K)III, and V(λ). Interestingly, V(L) family expression correlated with the cross-reactivity of these antibodies to the capsular carbohydrate of Escherichia coli K100. V(K)II antibodies did not cross-react, whereas antibodies expressing V(λ), V(K)I, or V(K)III generally cross-reacted. We conclude that L chain V regions are very important contributors to the limited heterogeneity in this antibody repertoire.

Original languageEnglish
Pages (from-to)293-298
Number of pages6
JournalJournal of Immunology
Volume143
Issue number1
StatePublished - Jan 1 1989

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