Clinical metagenomics for infectious corneal ulcers: Rags to riches?

Lawson Ung, Paulo J.M. Bispo, Thuy Doan, Russell N. Van Gelder, Michael S. Gilmore, Thomas Lietman, Todd P. Margolis, Michael E. Zegans, Cecilia S. Lee, James Chodosh

Research output: Contribution to journalEditorial

4 Scopus citations

Abstract

The emergence of clinical metagenomics as an unbiased, hypothesis-free approach to diagnostic testing is set to fundamentally alter the way infectious diseases are detected. Long envisioned as the solution to the limitations of culture-based conventional microbiology, next generation sequencing methods will soon mature, and our attention will inevitably turn to how they can be applied to areas of medicine which need it most urgently. In ophthalmology, the demand for this technology is particularly pressing for the care of infectious corneal ulcers, where current diagnostic tests may fail to identify a causative organism in over half of cases. However, the optimism found in the budding discourse surrounding clinical metagenomics belies the reality that clinicians and scientists will soon be inundated by oppressive volumes of sequencing data, much of which will be foreign and unfamiliar. Therefore, our success in translating clinical metagenomics is likely to hinge on how we make sense of these data, and understanding its implications for the interpretation and implementation of sequencing into routine clinical care. In this consortium-led review, we provide an outline of these data-related issues and how they may be used to inform technical workflows, with the hope that we may edge closer to realizing the potential of clinical metagenomics for this important unmet need.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalOcular Surface
Volume18
Issue number1
DOIs
StatePublished - Jan 2020

Keywords

  • Clinical metagenomics
  • Infectious corneal ulcers
  • Microbial keratitis
  • Next generation sequencing

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