Climbing the vertebrate branch of U1A/U2B″ protein evolution

Kimberly J. Delaney, Sandra G. Williams, Mariah Lawler, Kathleen B. Hall

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


In the vertebrate lineage of the U1A/U2B″/SNF protein family, the U1A and U2B″ proteins bind to RNA stem-loops in the U1 or U2 snRNPs, respectively. However, their specialization is fairly recent, as they evolved from a single ancestral protein. The progress of their specialization (subfunctionalization) can be monitored by the amino acid sequence changes that give rise to their modern RNA-binding specificity. Using ancestral sequence reconstruction to predict the intermediates on the evolutionary branch, a probable path of sequential changes is defined for U1A and U2B″. The RNA-binding affinity for U1A/U2B″ protein ancestors was measured using modern U1 and U2 snRNA stem-loops and RNA stem-loop variants to understand how the proteins' RNA specificities evolved.

Original languageEnglish
Pages (from-to)1035-1045
Number of pages11
Issue number7
StatePublished - Jul 2014


  • Ancestral reconstruction
  • RRM
  • RRM:RNA binding
  • U1A
  • snRNP


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