@article{40cfa56fb3c648498cf2ebcc759d752a,
title = "Climate-dependent variation in cold tolerance of weedy rice and rice mediated by OsICE1 promoter methylation",
abstract = "The mechanisms by which weedy rice (Oryza sativa f. spontanea) has adapted to endure low-temperature stress in northern latitudes remain unresolved. In this study, we assessed cold tolerance of 100 rice varieties and 100 co-occurring weedy rice populations, which were sampled across a broad range of climates in China. A parallel pattern of latitude-dependent variation in cold tolerance was detected in cultivated rice and weedy rice. At the molecular level, differential cold tolerance was strongly correlated with relative expression levels of CBF cold response pathway genes and with methylation levels in the promoter region of OsICE1, a regulator of this pathway. Among all methylated cytosine sites of the OsICE1 promoter, levels of CHG and CHH methylation were found to be significantly correlated with cold tolerance among accessions. Furthermore, within many of the collection locales, weedy rice shared identical or near-identical OsICE1 methylation patterns with co-occurring cultivated rice. These findings provide new insights on the possible roles that methylation variation in the OsICE1 promoter may play in cold tolerance, and they suggest that weedy rice can rapidly acquire cold tolerance via methylation patterns that are shared with co-occurring rice cultivars.",
keywords = "CBF pathway, DNA methylation, OsICE1, climatic adaptation, cold tolerance, weedy rice",
author = "Hongjie Xie and Yihao Han and Xinyue Li and Weimin Dai and Xiaoling Song and Olsen, {Kenneth M.} and Sheng Qiang",
note = "Funding Information: This study was supported by the National Natural Science Foundation of China (31572066) and the China Transgenic Organism Research and Commercialization Project (2016ZX08011-001). We thank Prof. Shushun Li, Dr. Lianju Zhang, Dr. Zheng Zhang, Dr. Dan Cao, Dr. Chaobin Zhang, Dr. Ranlin Zuo, Mr. Seong-Mu Kim, Mr. Kun Cai, Mr. Guanghui Sun, Mr. Xiaofeng Chen, Mr. Pinglei Gao, Mr. Can Zhao, Mrs. Rui Liu, Mrs. Mengrou Jiang and Mr. Hu Zhang from the Weed Research Laboratory of Nanjing Agricultural University for collecting the weedy rice and rice samples. We thank Prof. Mingshan Ji (Shenyang Agricultural University, China) for collecting samples from the Heilongjiang, Jilin and Liaoning provinces. We thank Prof. Shoubiao Zhou (Anhui Normal University, China) and staff of the Anhui Plant Protection and Quarantine Station for collecting samples from Anhui Province. We thank staff of the Henan Plant Protection and Quarantine Station for collecting samples from Henan Province. Funding Information: This study was supported by the National Natural Science Foundation of China (31572066) and the China Transgenic Organism Research and Commercialization Project (2016ZX08011‐001). We thank Prof. Shushun Li, Dr. Lianju Zhang, Dr. Zheng Zhang, Dr. Dan Cao, Dr. Chaobin Zhang, Dr. Ranlin Zuo, Mr. Seong‐Mu Kim, Mr. Kun Cai, Mr. Guanghui Sun, Mr. Xiaofeng Chen, Mr. Pinglei Gao, Mr. Can Zhao, Mrs. Rui Liu, Mrs. Mengrou Jiang and Mr. Hu Zhang from the Weed Research Laboratory of Nanjing Agricultural University for collecting the weedy rice and rice samples. We thank Prof. Mingshan Ji (Shenyang Agricultural University, China) for collecting samples from the Heilongjiang, Jilin and Liaoning provinces. We thank Prof. Shoubiao Zhou (Anhui Normal University, China) and staff of the Anhui Plant Protection and Quarantine Station for collecting samples from Anhui Province. We thank staff of the Henan Plant Protection and Quarantine Station for collecting samples from Henan Province. Publisher Copyright: {\textcopyright} 2019 John Wiley & Sons Ltd",
year = "2020",
month = jan,
day = "1",
doi = "10.1111/mec.15305",
language = "English",
volume = "29",
pages = "121--137",
journal = "Molecular Ecology",
issn = "0962-1083",
number = "1",
}