TY - JOUR
T1 - Classification of Indeterminate Melanocytic Lesions by MicroRNA Profiling
AU - Latchana, Nicholas
AU - del Campo, Sara E.Martin
AU - Grignol, Valerie P.
AU - Clark, Jennifer R.
AU - Albert, Scott P.
AU - Zhang, Jie
AU - Wei, Lai
AU - Aldrink, Jennifer H.
AU - Nicol, Kathleen K.
AU - Ranalli, Mark A.
AU - Peters, Sara B.
AU - Gru, Alejandro
AU - Trihka, Prashant
AU - Payne, Philip R.O.
AU - Howard, J. Harrison
AU - Carson, William E.
N1 - Funding Information:
This study was supported by National Institutes of Health (NIH) Grants P01 CA095426, P30 CA16058 and T32 CA009338 (to M. A. Caligiuri), and T32 CA090223 and K24 CA093670 (to W. E. Carson).
Publisher Copyright:
© 2016, Society of Surgical Oncology.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Purpose: Identification of indeterminate melanocytic skin lesions capable of neoplastic progression is suboptimal and may potentially result in unnecessary morbidity from surgery. MicroRNAs (miRs) may be useful in classifying indeterminate Spitz tumors as having high or low risk for malignant behavior. Methods: RNA was extracted from paraffin-embedded tissues of benign nevi, benign Spitz tumors, indeterminate Spitz tumors, and Spitzoid melanomas in adults (n = 62) and children (n = 28). The expression profile of 12 miRs in adults (6 miRs in children) was analyzed by real-time polymerase chain reaction. Results: Benign Spitz lesions were characterized by decreased expression of miR-125b and miR-211, and upregulation of miR-22, compared with benign nevi (p < 0.05). A comparison of Spitzoid melanomas to benign nevi revealed overexpression of miR-21, miR-150, and miR-155 in the malignant primaries (p < 0.05). In adults, Spitzoid melanomas exhibited upregulation of miR-21, miR-150, and miR-155 compared with indeterminate Spitz lesions. Indeterminate Spitz lesions with low-risk pathologic features had lower miR-21 and miR-155 expression compared with Spitzoid melanoma tumors in adults (p < 0.05), while pathologic high-risk indeterminate Spitz lesions had increased levels of miR-200c expression compared with low-risk indeterminate lesions (p < 0.05). Pediatric Spitzoid melanomas exhibited increased miR-21 expression compared with indeterminate Spitz lesions (p < 0.05). Moreover, miR-155 expression was increased in indeterminate lesions with mitotic counts >1 and depth of invasion >1 mm, suggesting miR-155 expression is associated with histological characteristics. Conclusions: miR expression profiles can be measured in indeterminate Spitz tumors and correlate with markers of malignant potential.
AB - Purpose: Identification of indeterminate melanocytic skin lesions capable of neoplastic progression is suboptimal and may potentially result in unnecessary morbidity from surgery. MicroRNAs (miRs) may be useful in classifying indeterminate Spitz tumors as having high or low risk for malignant behavior. Methods: RNA was extracted from paraffin-embedded tissues of benign nevi, benign Spitz tumors, indeterminate Spitz tumors, and Spitzoid melanomas in adults (n = 62) and children (n = 28). The expression profile of 12 miRs in adults (6 miRs in children) was analyzed by real-time polymerase chain reaction. Results: Benign Spitz lesions were characterized by decreased expression of miR-125b and miR-211, and upregulation of miR-22, compared with benign nevi (p < 0.05). A comparison of Spitzoid melanomas to benign nevi revealed overexpression of miR-21, miR-150, and miR-155 in the malignant primaries (p < 0.05). In adults, Spitzoid melanomas exhibited upregulation of miR-21, miR-150, and miR-155 compared with indeterminate Spitz lesions. Indeterminate Spitz lesions with low-risk pathologic features had lower miR-21 and miR-155 expression compared with Spitzoid melanoma tumors in adults (p < 0.05), while pathologic high-risk indeterminate Spitz lesions had increased levels of miR-200c expression compared with low-risk indeterminate lesions (p < 0.05). Pediatric Spitzoid melanomas exhibited increased miR-21 expression compared with indeterminate Spitz lesions (p < 0.05). Moreover, miR-155 expression was increased in indeterminate lesions with mitotic counts >1 and depth of invasion >1 mm, suggesting miR-155 expression is associated with histological characteristics. Conclusions: miR expression profiles can be measured in indeterminate Spitz tumors and correlate with markers of malignant potential.
UR - http://www.scopus.com/inward/record.url?scp=84980009962&partnerID=8YFLogxK
U2 - 10.1245/s10434-016-5476-9
DO - 10.1245/s10434-016-5476-9
M3 - Article
C2 - 27469124
AN - SCOPUS:84980009962
SN - 1068-9265
VL - 24
SP - 347
EP - 354
JO - Annals of Surgical Oncology
JF - Annals of Surgical Oncology
IS - 2
ER -