TY - JOUR
T1 - Circadian expression of clock genes in mouse macrophages, dendritic cells, and B cells
AU - Silver, Adam C.
AU - Arjona, Alvaro
AU - Hughes, Michael E.
AU - Nitabach, Michael N.
AU - Fikrig, Erol
N1 - Funding Information:
We thank Manchuan Chen for technical assistance with the qPCR. This work was supported by National Institutes of Health Training Grant , T32 AI07019 (to A.S.) and in part by N01 HHSN272201100019C. E.F. is an investigator with the Howard Hughes Medical Institute.
PY - 2012/3
Y1 - 2012/3
N2 - In mammals, circadian and daily rhythms influence nearly all aspects of physiology, ranging from behavior to gene expression. Functional molecular clocks have been described in the murine spleen and splenic NK cells. The aim of our study was to investigate the existence of molecular clock mechanisms in other immune cells. Therefore, we measured the circadian changes in gene expression of clock genes (Per1, Per2, Bmal1, and Clock) and clock-controlled transcription factors (Rev-erbα and Dbp) in splenic enriched macrophages, dendritic cells, and B cells in both mice entrained to a light-dark cycle and under constant environmental conditions. Our study reveals the existence of functional molecular clock mechanisms in splenic macrophages, dendritic cells, and B cells.
AB - In mammals, circadian and daily rhythms influence nearly all aspects of physiology, ranging from behavior to gene expression. Functional molecular clocks have been described in the murine spleen and splenic NK cells. The aim of our study was to investigate the existence of molecular clock mechanisms in other immune cells. Therefore, we measured the circadian changes in gene expression of clock genes (Per1, Per2, Bmal1, and Clock) and clock-controlled transcription factors (Rev-erbα and Dbp) in splenic enriched macrophages, dendritic cells, and B cells in both mice entrained to a light-dark cycle and under constant environmental conditions. Our study reveals the existence of functional molecular clock mechanisms in splenic macrophages, dendritic cells, and B cells.
KW - B cells
KW - Circadian rhythm
KW - Dendritic cells
KW - Gene expression
KW - Macrophages
UR - http://www.scopus.com/inward/record.url?scp=84857191157&partnerID=8YFLogxK
U2 - 10.1016/j.bbi.2011.10.001
DO - 10.1016/j.bbi.2011.10.001
M3 - Article
C2 - 22019350
AN - SCOPUS:84857191157
SN - 0889-1591
VL - 26
SP - 407
EP - 413
JO - Brain, Behavior, and Immunity
JF - Brain, Behavior, and Immunity
IS - 3
ER -