Chromosome-scale genome assembly provides insights into speciation of allotetraploid and massive biomass accumulation of elephant grass (Pennisetum purpureum Schum.)

Shengkui Zhang, Zhiqiang Xia, Can Li, Xiaohan Wang, Xianqin Lu, Wenqing Zhang, Haizhen Ma, Xincheng Zhou, Weixiong Zhang, Tingting Zhu, Pandao Liu, Guodao Liu, Wenquan Wang, Tao Xia

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Elephant grass (Pennisetum purpureum Schum) is an important forage, biofuels and industrial plant widely distributed in tropical and subtropical areas globally. It is characterized with robust growth and high biomass. We sequenced its allopolyploid genome and assembled 2.07 Gb into A’ and B subgenomes of 14 chromosomes with scaffold N50 of 8.47 Mb, yielding a total of 77,139 genes. The allotetraploid speciation occurred approximately 15 Ma after the divergence between Setaria italica and Pennisetum glaucum, according to a phylogenetic analysis of Pennisetum species. Double whole-genome duplication (WGD) and polyploidization events resulted in large-scale gene expansion, especially in the key steps of growth and biomass accumulation. Integrated transcriptome profiling revealed the functional divergence between subgenomes A’ and B. A’ subgenome mainly contributed to plant growth, development and photosynthesis, whereas the B subgenome was primarily responsible for effective transportation and resistance to stimulation. Some key gene families related to cellulose biosynthesis were expanded and highly expressed in stems, which could explain the high cellulose content in elephant grass. Our findings provide deep insights into genetic evolution of elephant grass and will aid future biological research and breeding, even for other grasses in the family Poaceae.

Original languageEnglish
Pages (from-to)2363-2378
Number of pages16
JournalMolecular Ecology Resources
Volume22
Issue number6
DOIs
StatePublished - Aug 2022

Keywords

  • Pennisetum purpureum
  • cellulose and lignin synthesis
  • de novo assembly
  • phylogenetic analysis
  • subgenomic divergence

Fingerprint

Dive into the research topics of 'Chromosome-scale genome assembly provides insights into speciation of allotetraploid and massive biomass accumulation of elephant grass (Pennisetum purpureum Schum.)'. Together they form a unique fingerprint.

Cite this