Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2)

John R. Bermingham, Karen C. Arden, Anna K. Naumova, Carmen Sapienza, Carrie S. Viars, X. D. Fu, Jhumku Khotz, James L. Manley, M. G. Rosenfeld

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The mammalian SR-type splicing factors ASF/SF2 and SC-35 play crucial roles in pre-mRNA splicing and have been shown to shift splice site choice in vitro. We have mapped the ASF/SF2 gene in mice and humans and the SC-35 gene in mice. Somatic cell hybrid mapping of the human ASF/SF2 gene (SFRS1 locus) reveals that it resides on chromosome 17, and fluorescence in situ hybridization refines this localization to 17q21.3-q22. Recombinant inbred mapping of the mouse ASF/SF2 gene (Sfrs1 locus) and the mouse SC-35 gene (Sfrs2 locus) demonstrates that both genes are located in a part of mouse chromosome 11 that is homologous to human chromosome 17. Mapping of Sfrs1 using F1 hybrid backcross mice between the strains C57BL/6 and DDK places Sfrs1 very near the marker D11Mit38 and indicates that the ASF/SF2 gene is closely linked to the Ovum mutant locus.

Original languageEnglish
Pages (from-to)70-79
Number of pages10
JournalGenomics
Volume29
Issue number1
DOIs
StatePublished - Sep 1 1995

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