Abstract

Chromatin immunoprecipitation (ChIP) is a powerful tool to study protein-DNA interaction and is widely used in many fields to study proteins associated with chromatin, such as histone and its isoforms and transcription factors, across a defined DNA domain. Here, we show the step-by-step methods currently used in our lab to immunoprecipitate the formaldehyde crosslinked chromatin and further analyze the immuprecipitated DNA by semiquantitative PCR.

Original languageEnglish
Pages (from-to)9-19
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume287
StatePublished - 2004

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