@inbook{93887e914fa24898a9d67e526efdfdee,
title = "Choroid plexus epithelial cells as a model to study nongenomic steroid signaling and its effect on ion channel function",
abstract = "The choroid plexus (CP) is an epithelial tissue primarily responsible for the secretion of the cerebrospinal fluid (CSF). Choroid plexuses are found in each of the four brain ventricles: two laterals, third and fourth. They ensure continuous production of CSF to provide nutrients, remove waste products and provide a mechanical buffer to protect the brain. Tight junctions in the CP epithelium form a barrier between the blood plasma and the CSF, which allow channels and transporters in the CP to establish a highly regulated concentration gradient of ions between the two fluids, thereby controlling the composition of CSF. CP plays an important part in healthy brain homeostasis, as its failure to maintain adequate CSF perfusion is implicated in Alzheimer's disease and traumatic brain injury. And yet, the physiology of CP and the mechanism of its age-related functional decline is one of the most understudied areas of neurobiology. Here, we describe a protocol to isolate and identify individual choroid plexus epithelial cells (CPEC) from murine brain for whole-cell patch-clamp recordings and ion channel identification. Using the recording from the inwardly rectifying potassium channel Kir7.1 and TRPM3 that are abundant in CP, we demonstrate a technique to study the regulators of ion channels in the choroid plexus.",
keywords = "Cerebrospinal fluid, Choroid plexus, Choroid plexus epithelial cells, Ion channels, K7.1, Steroid hormones",
author = "Monika Haoui and Petersen, {Natalie True} and Ida Bj{\"o}rkgren and Chung, {Dong Hwa} and Lishko, {Polina V.}",
note = "Funding Information: This project was funded in part by grant number GCRLE-0920 from the Global Consortium for Reproductive Longevity and Equality at the Buck Institute for Research on Aging, made possible by the Bia-Echo Foundation, Pew Biomedical Scholars Award and Packer Wentz endowment will (to P.V.L.). Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = jan,
doi = "10.1016/bs.mie.2021.03.004",
language = "English",
isbn = "9780128239247",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "297--314",
editor = "Minor, {Daniel L.} and Colecraft, {Henry M.}",
booktitle = "Ion Channels",
}