@inbook{5096c47f75154fed8f8b537ec0770949,
title = "Chondrogenic Differentiation of Human-Induced Pluripotent Stem Cells",
abstract = "The generation of large quantities of genetically defined human chondrocytes remains a critical step for the development of tissue engineering strategies for cartilage regeneration and high-throughput drug screening. This protocol describes chondrogenic differentiation of human-induced pluripotent stem cells (hiPSCs), which can undergo genetic modification and the capacity for extensive cell expansion. The hiPSCs are differentiated in a stepwise manner in monolayer through the mesodermal lineage for 12 days using defined growth factors and small molecules. This is followed by 28 days of chondrogenic differentiation in a 3D pellet culture system using transforming growth factor beta 3 and specific compounds to inhibit off-target differentiation. The 6-week protocol results in hiPSC-derived cartilaginous tissue that can be characterized by histology, immunohistochemistry, and gene expression or enzymatically digested to isolate chondrocyte-like cells. Investigators can use this protocol for experiments including genetic engineering, in vitro disease modeling, or tissue engineering.",
keywords = "Chondrogenesis, Human iPSCs, Stem cells, Tissue-engineered cartilage",
author = "Dicks, {Amanda R.} and Nancy Steward and Farshid Guilak and Wu, {Chia Lung}",
note = "Funding Information: This work was supported by the Shriners Hospitals for Children, Nancy Taylor Foundation, Arthritis Foundation, NIH (AG46927, AG15768, AR075899, AR072999, AR074992, AR073752, AR080902, T32 DK108742, T32 EB018266), and Taiwan GSSA Scholarship. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2839-3_8",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "87--114",
booktitle = "Methods in Molecular Biology",
}