Cholinergic imbalance in lumbar spinal cord of a rat model of multiple sclerosis

Chunling Liu; Hui Liu; Hongjun Jin; Xuyi Yue; Zonghua Luo; Zhude Tu

doi:10.1016/j.jneuroim.2018.01.016

Cholinergic imbalance in lumbar spinal cord of a rat model of multiple sclerosis

Chunling Liu, Hui Liu, Hongjun Jin, Xuyi Yue, Zonghua Luo, Zhude Tu

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Cholinergic dysfunction in the central nervous system is an important characteristic of multiple sclerosis and experimental autoimmune encephalomyelitis (EAE). By using a rat EAE model, upregulation of vesicular acetylcholine transporter (VAChT) level in the EAE rat lumbar spinal cord was detected by western blot and immunostaining, and was associated with lymphocyte filtration and glial activation. Ex vivo and in vitro autoradiography studies with [¹⁸F]VAT, a VAChT-specific radioligand, also revealed increased tracer uptake in EAE rat lumbar spinal cord compared with shams. These studies on VAChT expression suggest central cholinergic imbalance during EAE progression.

Original language	English
Pages (from-to)	29-35
Number of pages	7
Journal	Journal of Neuroimmunology
Volume	318
DOIs	https://doi.org/10.1016/j.jneuroim.2018.01.016
State	Published - May 15 2018

Keywords

Experimental autoimmune encephalomyelitis
Neuroinflammation
Vesicular acetylcholine transporter

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10.1016/j.jneuroim.2018.01.016

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title = "Cholinergic imbalance in lumbar spinal cord of a rat model of multiple sclerosis",

abstract = "Cholinergic dysfunction in the central nervous system is an important characteristic of multiple sclerosis and experimental autoimmune encephalomyelitis (EAE). By using a rat EAE model, upregulation of vesicular acetylcholine transporter (VAChT) level in the EAE rat lumbar spinal cord was detected by western blot and immunostaining, and was associated with lymphocyte filtration and glial activation. Ex vivo and in vitro autoradiography studies with [18F]VAT, a VAChT-specific radioligand, also revealed increased tracer uptake in EAE rat lumbar spinal cord compared with shams. These studies on VAChT expression suggest central cholinergic imbalance during EAE progression.",

keywords = "Experimental autoimmune encephalomyelitis, Neuroinflammation, Vesicular acetylcholine transporter",

author = "Chunling Liu and Hui Liu and Hongjun Jin and Xuyi Yue and Zonghua Luo and Zhude Tu",

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AU - Liu, Chunling

AU - Liu, Hui

AU - Jin, Hongjun

AU - Yue, Xuyi

AU - Luo, Zonghua

AU - Tu, Zhude

PY - 2018/5/15

Y1 - 2018/5/15

N2 - Cholinergic dysfunction in the central nervous system is an important characteristic of multiple sclerosis and experimental autoimmune encephalomyelitis (EAE). By using a rat EAE model, upregulation of vesicular acetylcholine transporter (VAChT) level in the EAE rat lumbar spinal cord was detected by western blot and immunostaining, and was associated with lymphocyte filtration and glial activation. Ex vivo and in vitro autoradiography studies with [18F]VAT, a VAChT-specific radioligand, also revealed increased tracer uptake in EAE rat lumbar spinal cord compared with shams. These studies on VAChT expression suggest central cholinergic imbalance during EAE progression.

AB - Cholinergic dysfunction in the central nervous system is an important characteristic of multiple sclerosis and experimental autoimmune encephalomyelitis (EAE). By using a rat EAE model, upregulation of vesicular acetylcholine transporter (VAChT) level in the EAE rat lumbar spinal cord was detected by western blot and immunostaining, and was associated with lymphocyte filtration and glial activation. Ex vivo and in vitro autoradiography studies with [18F]VAT, a VAChT-specific radioligand, also revealed increased tracer uptake in EAE rat lumbar spinal cord compared with shams. These studies on VAChT expression suggest central cholinergic imbalance during EAE progression.

KW - Experimental autoimmune encephalomyelitis

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ER -

Cholinergic imbalance in lumbar spinal cord of a rat model of multiple sclerosis

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Keywords

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