TY - JOUR
T1 - Cholesterol dependence of HTLV-I infection
AU - Wielgosz, Matthew M.
AU - Rauch, Daniel A.
AU - Jones, Kathryn S.
AU - Ruscetti, Frank W.
AU - Ratner, Lee
PY - 2005/1
Y1 - 2005/1
N2 - Cholesterol-rich plasma membrane microdomains are important for entry of many viruses, including retroviruses. Depletion of cholesterol with 2-hydroxypropyl-β-cyclodexirin inhibits entry of human T cell leukemia virus type I (HTLV-1) and HTLV-I envelope pseudotyped lentivirus particles. Using a soluble fusion protein of the HTLV-I surface envelope protein with the immunoglobulin Fc domain, the HTLV-I receptor was found to colocalize with a raft-associated marker and to cluster in specific plasma membrane microdomains. Depletion of cholesterol did not alter receptor binding activity, suggesting a requirement for cholesterol in a postbinding virus entry step.
AB - Cholesterol-rich plasma membrane microdomains are important for entry of many viruses, including retroviruses. Depletion of cholesterol with 2-hydroxypropyl-β-cyclodexirin inhibits entry of human T cell leukemia virus type I (HTLV-1) and HTLV-I envelope pseudotyped lentivirus particles. Using a soluble fusion protein of the HTLV-I surface envelope protein with the immunoglobulin Fc domain, the HTLV-I receptor was found to colocalize with a raft-associated marker and to cluster in specific plasma membrane microdomains. Depletion of cholesterol did not alter receptor binding activity, suggesting a requirement for cholesterol in a postbinding virus entry step.
UR - http://www.scopus.com/inward/record.url?scp=13144305052&partnerID=8YFLogxK
U2 - 10.1089/aid.2005.21.43
DO - 10.1089/aid.2005.21.43
M3 - Article
C2 - 15665643
AN - SCOPUS:13144305052
SN - 0889-2229
VL - 21
SP - 43
EP - 50
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
IS - 1
ER -