Cholera toxin B conjugated quantum dots for live cell labeling

Subhasish K. Chakraborty, James A.J. Fitzpatrick, Julie A. Phillippi, Susan Andreko, Alan S. Waggoner, Marcel P. Bruchez, Byron Ballou

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

Cholera toxin subunit B (CTB)-quantum dot conjugates were developed for labeling mammalian cells. The conjugates were internalized by all tested cell lines into small vesicles dispersed throughout the cytoplasm, while commercially available polyarginine conjugates rapidly accumulated in large perinuclear endosomes. Although a large proportion of CTB conjugates eventually also accumulated in perinuclear endosomes, this accumulation required several days, and even then many CTB conjugated quantum dots remained in small vesicles dispersed throughout the cytoplasm. Thus CTB conjugates are a practical alternative to polyarginine conjugates for the general labeling of mammalian cells.

Original languageEnglish
Pages (from-to)2618-2626
Number of pages9
JournalNano Letters
Volume7
Issue number9
DOIs
StatePublished - Sep 1 2007
Externally publishedYes

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  • Cite this

    Chakraborty, S. K., Fitzpatrick, J. A. J., Phillippi, J. A., Andreko, S., Waggoner, A. S., Bruchez, M. P., & Ballou, B. (2007). Cholera toxin B conjugated quantum dots for live cell labeling. Nano Letters, 7(9), 2618-2626. https://doi.org/10.1021/nl0709930