Abstract
125I-Mannose-BSA is taken up by macrophages by receptor-mediated pinocytosis. Previous studies indicated that uptake is rapid and proceeds linearly with time in the absence of protein synthesis suggesting that receptors are conserved and recycled. Chloroquine and NH4+ inhibit uptake of 125I-Mannose-BSA and its subsequent digestion by macrophages. Inhibition of uptake is inhibitor-concentration and time dependent. Both binding of ligand (4°) and internalization of pre-bound ligand (4°) following warm-up are unaffected by the inhibitors. However, brief incubation of cells at 37° with inhibitor in the absence of ligand results in a substantial reduction in cell surface binding sites. Our interpretation of these results is that chloroquine and NH4+ inhibit receptor recycling and thereby inhibit ligand uptake.
Original language | English |
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Pages (from-to) | 1-8 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 93 |
Issue number | 1 |
DOIs | |
State | Published - Mar 13 1980 |