Chinese hamster ovary cells produce sufficient recombinant insulin-like growth factor I to support growth in serum-free medium

S. M.N. Hunt, S. C.O. Pak, M. W. Bridges, P. P. Gray, M. J. Sleigh

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Insulin-like growth factor I has similar mitogenic effects to insulin, a growth factor required by most cells in culture, and it can replace insulin in serum-free formulations for some cells. Chinese Hamster Ovary cells grow well in serum-free medium with insulin and transferrin as the only exogenous growth factors. An alternative approach to addition of exogenous growth factors to serum-free medium is transfection of host cells with growth factor-encoding genes, permitting autocrine growth. Taking this approach, we constructed an IGF-I heterologous gene driven by the cytomegalovirus promoter, introduced it into Chinese Hamster Ovary cells and examined the growth characteristics of Insulin-like growth factor I-expressing clonal cells in the absence of the exogenous factor. The transfected cells secreted up to 500 ng/106 cells/day of mature Insulin-like growth factor I into the conditioned medium and as a result they grew autonomously in serum-free medium containing transferrin as the only added growth factor. This growth-stimulating effect, observed under both small and large scale culture conditions, was maximal since no further improvement was observed in the presence of exogenous insulin.

Original languageEnglish
Pages (from-to)55-64
Number of pages10
JournalCytotechnology
Volume24
Issue number1
DOIs
StatePublished - 1997

Keywords

  • CHO
  • IGF-I
  • autocrine growth
  • cell culture
  • serum-free

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