Chemogenetic isolation reveals synaptic contribution of δ GABA A receptors in mouse dentate granule neurons

Min Yu Sun, Hong Jin Shu, Ann Benz, John Bracamontes, Gustav Akk, Charles F. Zorumski, Joe Henry Steinbach, Steven J. Mennerick

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Two major GABA A receptor classes mediate ionotropic GABA signaling, those containing a δ subunit and those with a γ2 subunit. The classical viewpoint equates γ2-containing receptors with IPSCs and δ-containing receptors with tonic inhibition because of differences in receptor localization, but significant questions remain because the populations cannot be pharmacologically separated. We removed this barrier using gene editing to confer a point mutation on the δ subunit in mice, rendering receptors containing the subunit picrotoxin resistant. By pharmacologically isolating δ-containing receptors, our results demonstrate their contribution to IPSCs in dentate granule neurons and weaker contributions to thalamocortical IPSCs. Despite documented extrasynaptic localization, we found that receptor localization does not preclude participation in isolated IPSCs, including mIPSCs. Further, phasic inhibition from δ subunit-containing receptors strongly inhibited summation of EPSPs, whereas tonic activity had little impact. In addition to any role that δ-containing receptors may play in canonical tonic inhibition, our results highlight a previously underestimated contribution of δ-containing receptors to phasic inhibition.

Original languageEnglish
Pages (from-to)8128-8145
Number of pages18
JournalJournal of Neuroscience
Issue number38
StatePublished - Sep 19 2018


  • GABA
  • Hippocampus
  • IPSC
  • Inhibition
  • Picrotoxin
  • Tonic


Dive into the research topics of 'Chemogenetic isolation reveals synaptic contribution of δ GABA <sub>A</sub> receptors in mouse dentate granule neurons'. Together they form a unique fingerprint.

Cite this