Chemiluminescent-based methods to detect subpicomole levels of c-type cytochromes

Robert Feissner; Youbin Xiang; Robert G. Kranz

doi:10.1016/S0003-2697(02)00658-9

Chemiluminescent-based methods to detect subpicomole levels of c-type cytochromes

Robert Feissner, Youbin Xiang, Robert G. Kranz

Research output: Contribution to journal › Article › peer-review

77 Scopus citations

Abstract

A variety of luminol-based substrates and either an autoradiographic film or a charge-coupled device (CCD) imaging system were used for chemiluminescence detection of c-type cytochromes. The Pierce Femto peroxidase substrate was at least 10 times more sensitive when using film than the highly cited 3,3′,5,5′-tetramethylbenzidine (benzidine derivative) staining method and 50 times more sensitive when using a CCD imaging system. Film or CCD imaging result in highly sensitive and quantitative signals. The quantitative detection of c-type cytochromes from single colonies or from less than 1ml of a bacterial culture is possible.

Original language	English
Pages (from-to)	90-94
Number of pages	5
Journal	Analytical Biochemistry
Volume	315
Issue number	1
DOIs	https://doi.org/10.1016/S0003-2697(02)00658-9
State	Published - Apr 1 2003

Keywords

Chemiluminescence
Cytochrome c
Detection
Heme protein

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10.1016/S0003-2697(02)00658-9

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abstract = "A variety of luminol-based substrates and either an autoradiographic film or a charge-coupled device (CCD) imaging system were used for chemiluminescence detection of c-type cytochromes. The Pierce Femto peroxidase substrate was at least 10 times more sensitive when using film than the highly cited 3,3′,5,5′-tetramethylbenzidine (benzidine derivative) staining method and 50 times more sensitive when using a CCD imaging system. Film or CCD imaging result in highly sensitive and quantitative signals. The quantitative detection of c-type cytochromes from single colonies or from less than 1ml of a bacterial culture is possible.",

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AU - Kranz, Robert G.

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N2 - A variety of luminol-based substrates and either an autoradiographic film or a charge-coupled device (CCD) imaging system were used for chemiluminescence detection of c-type cytochromes. The Pierce Femto peroxidase substrate was at least 10 times more sensitive when using film than the highly cited 3,3′,5,5′-tetramethylbenzidine (benzidine derivative) staining method and 50 times more sensitive when using a CCD imaging system. Film or CCD imaging result in highly sensitive and quantitative signals. The quantitative detection of c-type cytochromes from single colonies or from less than 1ml of a bacterial culture is possible.

AB - A variety of luminol-based substrates and either an autoradiographic film or a charge-coupled device (CCD) imaging system were used for chemiluminescence detection of c-type cytochromes. The Pierce Femto peroxidase substrate was at least 10 times more sensitive when using film than the highly cited 3,3′,5,5′-tetramethylbenzidine (benzidine derivative) staining method and 50 times more sensitive when using a CCD imaging system. Film or CCD imaging result in highly sensitive and quantitative signals. The quantitative detection of c-type cytochromes from single colonies or from less than 1ml of a bacterial culture is possible.

KW - Chemiluminescence

KW - Cytochrome c

KW - Detection

KW - Heme protein

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JO - Analytical Biochemistry

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ER -

Chemiluminescent-based methods to detect subpicomole levels of c-type cytochromes

Abstract

Keywords

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