Chemical inhibition of the auxin inactivation pathway uncovers the roles of metabolic turnover in auxin homeostasis

Kosuke Fukui, Kazushi Arai, Yuka Tanaka, Yuki Aoi, Vandna Kukshal, Joseph M. Jez, Martin F. Kubes, Richard Napier, Yunde Zhao, Hiroyuki Kasahara, Ken Ichiro Hayashi

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9 Scopus citations

Abstract

The phytohormone auxin, indole-3-acetic acid (IAA), plays a prominent role in plant development. Auxin homeostasis is coordinately regulated by auxin synthesis, transport, and inactivation; however, the physiological contribution of auxin inactivation to auxin homeostasis has not been determined. The GH3 IAA-amino acid conjugating enzymes play a central role in auxin inactivation. Chemical inhibition of GH3 proteins in planta is challenging because the inhibition of these enzymes leads to IAA overaccumulation that rapidly induces GH3 expression. Here, we report the characterization of a potent GH3 inhibitor, kakeimide, that selectively targets IAA-conjugating GH3 proteins. Chemical knockdown of the auxin inactivation pathway demonstrates that auxin turnover is very rapid (about 10 min) and indicates that both auxin biosynthesis and inactivation dynamically regulate auxin homeostasis.

Original languageEnglish
Article numbere2206869119
JournalProceedings of the National Academy of Sciences of the United States of America
Volume119
Issue number32
DOIs
StatePublished - Aug 9 2022

Keywords

  • auxin
  • chemical biology
  • plant hormone

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