Characterizing the chromosomes of the platypus (Ornithorhynchus anatinus)

Daniel McMillan; Pat Miethke; Amber E. Alsop; Willem Rens; Patricia O'Brien; Vladimir Trifonov; Frederic Veyrunes; Kyriena Schatzkamer; Colin L. Kremitzki; Tina Graves; Wesley Warren; Frank Grützner; Malcolm A. Ferguson-Smith; Jennifer A.Marshall Graves

doi:10.1007/s10577-007-1186-2

Characterizing the chromosomes of the platypus (Ornithorhynchus anatinus)

Daniel McMillan, Pat Miethke, Amber E. Alsop, Willem Rens, Patricia O'Brien, Vladimir Trifonov, Frederic Veyrunes, Kyriena Schatzkamer, Colin L. Kremitzki, Tina Graves, Wesley Warren, Frank Grützner, Malcolm A. Ferguson-Smith, Jennifer A.Marshall Graves

McDonnell Genome Institute (MGI)

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Like the unique platypus itself, the platypus genome is extraordinary because of its complex sex chromosome system, and is controversial because of difficulties in identification of small autosomes and sex chromosomes. A 6-fold shotgun sequence of the platypus genome is now available and is being assembled with the help of physical mapping. It is therefore essential to characterize the chromosomes and resolve the ambiguities and inconsistencies in identifying autosomes and sex chromosomes. We have used chromosome paints and DAPI banding to identify and classify pairs of autosomes and sex chromosomes. We have established an agreed nomenclature and identified anchor BAC clones for each chromosome that will ensure unambiguous gene localizations.

Original language	English
Pages (from-to)	961-974
Number of pages	14
Journal	Chromosome Research
Volume	15
Issue number	8
DOIs	https://doi.org/10.1007/s10577-007-1186-2
State	Published - Dec 2007

Keywords

Chromosome painting
Karyology
Ornithorhynchus anatinus
Platypus
Sex chromosomes

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10.1007/s10577-007-1186-2

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title = "Characterizing the chromosomes of the platypus (Ornithorhynchus anatinus)",

abstract = "Like the unique platypus itself, the platypus genome is extraordinary because of its complex sex chromosome system, and is controversial because of difficulties in identification of small autosomes and sex chromosomes. A 6-fold shotgun sequence of the platypus genome is now available and is being assembled with the help of physical mapping. It is therefore essential to characterize the chromosomes and resolve the ambiguities and inconsistencies in identifying autosomes and sex chromosomes. We have used chromosome paints and DAPI banding to identify and classify pairs of autosomes and sex chromosomes. We have established an agreed nomenclature and identified anchor BAC clones for each chromosome that will ensure unambiguous gene localizations.",

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AU - McMillan, Daniel

AU - Miethke, Pat

AU - Alsop, Amber E.

AU - Rens, Willem

AU - O'Brien, Patricia

AU - Trifonov, Vladimir

AU - Veyrunes, Frederic

AU - Schatzkamer, Kyriena

AU - Kremitzki, Colin L.

AU - Graves, Tina

AU - Warren, Wesley

AU - Grützner, Frank

AU - Ferguson-Smith, Malcolm A.

AU - Graves, Jennifer A.Marshall

PY - 2007/12

Y1 - 2007/12

N2 - Like the unique platypus itself, the platypus genome is extraordinary because of its complex sex chromosome system, and is controversial because of difficulties in identification of small autosomes and sex chromosomes. A 6-fold shotgun sequence of the platypus genome is now available and is being assembled with the help of physical mapping. It is therefore essential to characterize the chromosomes and resolve the ambiguities and inconsistencies in identifying autosomes and sex chromosomes. We have used chromosome paints and DAPI banding to identify and classify pairs of autosomes and sex chromosomes. We have established an agreed nomenclature and identified anchor BAC clones for each chromosome that will ensure unambiguous gene localizations.

AB - Like the unique platypus itself, the platypus genome is extraordinary because of its complex sex chromosome system, and is controversial because of difficulties in identification of small autosomes and sex chromosomes. A 6-fold shotgun sequence of the platypus genome is now available and is being assembled with the help of physical mapping. It is therefore essential to characterize the chromosomes and resolve the ambiguities and inconsistencies in identifying autosomes and sex chromosomes. We have used chromosome paints and DAPI banding to identify and classify pairs of autosomes and sex chromosomes. We have established an agreed nomenclature and identified anchor BAC clones for each chromosome that will ensure unambiguous gene localizations.

KW - Chromosome painting

KW - Karyology

KW - Ornithorhynchus anatinus

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Characterizing the chromosomes of the platypus (Ornithorhynchus anatinus)

Abstract

Keywords

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