TY - JOUR
T1 - Characterization of the human homolog of the IL-4 induced gene-1 (Fig1)
AU - Chavan, Sangeeta S.
AU - Tian, Wenzhi
AU - Hsueh, Kevin
AU - Jawaheer, Damini
AU - Gregersen, Peter K.
AU - Chu, Charles C.
N1 - Funding Information:
We thank Dr. Robert Pergolizzi, Dorothy Guzowski, Colleen Millan, and Craig Gawel for oligonucleotide synthesis and DNA sequencing; and Diana Chang, Dr. Nicholas Chiorazzi, and Dr. Mamta Naidu for critical review, support, and encouragement. This work was supported by the Muriel Fusfeld Foundation, Leonard Wagner Autoimmunity Fund, S.L.E. Foundation, Inc., and the National Institutes of Health (AI44837-01).
PY - 2002/6/7
Y1 - 2002/6/7
N2 - Mouse interleukin-four induced gene-1 (mFig1) maps to a region of susceptibility for systemic lupus erythematosus (SLE) that includes the Sle3 locus. To begin examining this relationship in humans, we have isolated and characterized the human homolog of mFig1. Human Fig1 (hFig1) has the same eight exon genomic structure as mFig1. The predicted 63-kDa protein, like mFig1, contains a signal peptide, a large internal sequence that is most similar (43% identical over 484 amino acids) to L-amino acid oxidase (LAAO), and a carboxy terminal domain with no similarity to known genes. When compared to the LAAO crystal structure, hFig1 conserves key residues thought to be involved in catalysis and binding of the flavin adenine dinucleotide cofactor. Surprisingly, the carboxy terminal domains of hFig1 and mFig1 have little similarity (<11% identity), different lengths and amino acid composition. Like mFig1, hFig1 RNA is induced by interleukin-4 (IL-4) in B lymphocytes, and is primarily found in immune tissues. Finally, hFig1 maps to the predicted mFig1 syntenic region on human chromosome 19q13.3-19q13.4, a hot spot for susceptibility to several autoimmune diseases, including SLE.
AB - Mouse interleukin-four induced gene-1 (mFig1) maps to a region of susceptibility for systemic lupus erythematosus (SLE) that includes the Sle3 locus. To begin examining this relationship in humans, we have isolated and characterized the human homolog of mFig1. Human Fig1 (hFig1) has the same eight exon genomic structure as mFig1. The predicted 63-kDa protein, like mFig1, contains a signal peptide, a large internal sequence that is most similar (43% identical over 484 amino acids) to L-amino acid oxidase (LAAO), and a carboxy terminal domain with no similarity to known genes. When compared to the LAAO crystal structure, hFig1 conserves key residues thought to be involved in catalysis and binding of the flavin adenine dinucleotide cofactor. Surprisingly, the carboxy terminal domains of hFig1 and mFig1 have little similarity (<11% identity), different lengths and amino acid composition. Like mFig1, hFig1 RNA is induced by interleukin-4 (IL-4) in B lymphocytes, and is primarily found in immune tissues. Finally, hFig1 maps to the predicted mFig1 syntenic region on human chromosome 19q13.3-19q13.4, a hot spot for susceptibility to several autoimmune diseases, including SLE.
KW - Autoimmunity
KW - B lymphocyte
KW - Cytokine
KW - Human
KW - Interleukin-four
KW - Systemic lupus erythematosus
UR - http://www.scopus.com/inward/record.url?scp=0037036119&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(02)00295-6
DO - 10.1016/S0167-4781(02)00295-6
M3 - Article
C2 - 12031486
AN - SCOPUS:0037036119
SN - 0167-4781
VL - 1576
SP - 70
EP - 80
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 1-2
ER -