TY - JOUR
T1 - Characterization of the heterotrimeric G-protein family and its transmembrane regulator from capsicum (Capsicum annuum L.)
AU - Romero-Castillo, Rafael A.
AU - Roy Choudhury, Swarup
AU - León-Félix, Josefina
AU - Pandey, Sona
N1 - Funding Information:
This work was supported by the Mexican National Council for Science and Technology (scholarship number 491894 to R.A. R-C. and J. L-F.), the Mexican Basic Science Project (No. CB183180 to R.A. R-C. and J. L-F.), and the National Science Foundation , USA (MCB-1157944 to S.P.).
Publisher Copyright:
© 2015 Elsevier Ireland Ltd.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Throughout evolution, organisms have created numerous mechanisms to sense and respond to their environment. One such highly conserved mechanism involves regulation by heterotrimeric G-protein complex comprised of alpha (Gα), beta (Gβ) and gamma (Gγ) subunits. In plants, these proteins play important roles in signal transduction pathways related to growth and development including response to biotic and abiotic stresses and consequently affect yield. In this work, we have identified and characterized the complete heterotrimeric G-protein repertoire in the Capsicum annuum (Capsicum) genome which consists of one Gα, one Gβ and three Gγ genes. We have also identified one RGS gene in the Capsicum genome that acts as a regulator of the G-protein signaling. Biochemical activities of the proteins were confirmed by assessing the GTP-binding and GTPase activity of the recombinant Gα protein and its regulation by the GTPase acceleration activity of the RGS protein. Interaction between different subunits was established using yeast- and plant-based analyses. Gene and protein expression profiles of specific G-protein components revealed interesting spatial and temporal regulation patterns, especially during root development and during fruit development and maturation. This research thus details the characterization of the first heterotrimeric G-protein family from a domesticated, commercially important vegetable crop.
AB - Throughout evolution, organisms have created numerous mechanisms to sense and respond to their environment. One such highly conserved mechanism involves regulation by heterotrimeric G-protein complex comprised of alpha (Gα), beta (Gβ) and gamma (Gγ) subunits. In plants, these proteins play important roles in signal transduction pathways related to growth and development including response to biotic and abiotic stresses and consequently affect yield. In this work, we have identified and characterized the complete heterotrimeric G-protein repertoire in the Capsicum annuum (Capsicum) genome which consists of one Gα, one Gβ and three Gγ genes. We have also identified one RGS gene in the Capsicum genome that acts as a regulator of the G-protein signaling. Biochemical activities of the proteins were confirmed by assessing the GTP-binding and GTPase activity of the recombinant Gα protein and its regulation by the GTPase acceleration activity of the RGS protein. Interaction between different subunits was established using yeast- and plant-based analyses. Gene and protein expression profiles of specific G-protein components revealed interesting spatial and temporal regulation patterns, especially during root development and during fruit development and maturation. This research thus details the characterization of the first heterotrimeric G-protein family from a domesticated, commercially important vegetable crop.
KW - Capsicum annuum
KW - Fruit development
KW - Heterotrimeric g-proteins
KW - Pepper g-proteins
KW - Protein-protein interaction
KW - Regulator of g protein signaling
UR - http://www.scopus.com/inward/record.url?scp=84924027006&partnerID=8YFLogxK
U2 - 10.1016/j.plantsci.2015.02.007
DO - 10.1016/j.plantsci.2015.02.007
M3 - Article
C2 - 25804813
AN - SCOPUS:84924027006
SN - 0168-9452
VL - 234
SP - 97
EP - 109
JO - Plant Science
JF - Plant Science
ER -