Characterization of the gene encoding mouse retinoblastoma binding protein-7, a component of chromatin-remodeling complexes

Jing Yang, Susan Mc Leskey Kiefer, Michael Rauchman

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

RBBP7 is a highly conserved WD-repeat protein that interacts with histone deacetylases and is a component of several co-repressor complexes. The mouse gene Rbbp7 spans ∼ 20 kb, consists of at least 12 exons, and contains a C/T polymorphism in the 3′ splice acceptor region of intron 3. We found that Rbbp7 contains a TATA-less promoter with multiple transcription initiation sites. In transient transfection assays, we identified potential positive regulatory elements upstream of the proximal promoter at -668 to -1710. RBBP7 protein is detectable from at least day 9.5 of embryogenesis and is strongly expressed in the developing kidney and brain. Consistent with its association with co-repressor complexes, we demonstrate that RBBP7 represses the c-FOS transactivation domain in response to mitogen stimulation. We have also excluded human RBBP7 as a candidate gene in six patients that exhibit X-linked mental retardation, a heterogeneous developmental disorder that has been linked in some cases to mutations in genes involved in chromatin remodeling.

Original languageEnglish
Pages (from-to)407-415
Number of pages9
JournalGenomics
Volume80
Issue number4
DOIs
StatePublished - Oct 2002

Keywords

  • CAF1
  • Chromatin remodeling
  • Histone deacetylase (HDAC)
  • LIN-53
  • NURD
  • RBA-1
  • Retinoblastoma binding protein 7 (RBBP7)
  • Retinoblastoma-associated protein 46 (RBAP46)
  • SIN3A
  • Transcriptional repression
  • X-linked mental retardation (XLMR)

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