Characterization of the α4 integrin gene promoter

Glenn D. Rosen, Thomas M. Birkenmeier, Douglas C. Dean

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

A cDNA for the α4 chain of the α4β1 integrin was described previously [Takada, Y., Elices, M. J., Crouse, C. & Hemler, M. E. (1989) EMBO J. 8, 1361-1368]. Primer extension analysis indicated that α4 mRNA extended well beyond the 5′ end of this cDNA. To clone this 5′ sequence, a primer extension cDNA library was constructed, and a cDNA extending an additional 660 base pairs was isolated. This cDNA hybridized to multiple mRNAs in both T and B lymphocytes, but no α4 mRNA was found in different tissues or in adherent cell lines. A single α4 gene was detected in a genomic Southern blot when hybridization was done at high stringency; however, additional bands were observed at lower stringency, indicating the presence of α4-related genes. Some of the different mRNAs that hybridize to the α4 cDN A may then be the products of these related genes. Analysis of the α4 genomic sequence revealed a large first exon of 958 base pairs. Interestingly, translation of α4 initiates from the second ATG in this exon (nucleotide +744). The first ATG (nucleotide +21) is followed by a termination codon 21 amino acids downstream. Such upstream ATG codons have been implicated in translational control of protooncogenes. One major transcriptional start site was identified by using S1 nuclease and primer extension mapping. Consensus sequences for DNA regulatory elements were found upstream of the gene and in exon 1 and intron 1. The α4 gene 5′ flanking region acted as a promoter in transfection assays. Detailed characterization of the promoter should provide insight into molecular events regulating expression and tissue specificity of α4.

Original languageEnglish
Pages (from-to)4094-4098
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number10
DOIs
StatePublished - May 15 1991

Keywords

  • DNA sequencing
  • DNA transfection
  • RNA mapping

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