Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging

Philip P. Cheney; Gregg B. Fields; Samuel Achilefu; W. Barry Edwards

doi:10.1117/12.810096

Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging

Philip P. Cheney, Gregg B. Fields, Samuel Achilefu, W. Barry Edwards

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

The prevalence of the gelatinases, MMP-2 and MMP-9, in many human tumors, including breast, colorectal, prostate and gastric cancer, make them an attractive target for molecular imaging. A self assembling homotrimeric triple helical peptide (THP), incorporating sequences from type V collagen with high specificity to MMP-2 and MMP-9, was previously developed. To investigate the viability of a THP for gelatinase imaging, we conjugated 5FAM to ε-amino groups of lysine flanking the hydrolysis site and subjected this substrate (THP-5FAM) to vitro analysis. The synthesis and in vitro results was presented.

Original language	English
Title of host publication	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
DOIs	https://doi.org/10.1117/12.810096
State	Published - Jun 15 2009
Event	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications - San Jose, CA, United States Duration: Jan 26 2009 → Jan 29 2009

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7190
ISSN (Print)	1605-7422

Conference

Conference	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
Country/Territory	United States
City	San Jose, CA
Period	01/26/09 → 01/29/09

Keywords

Cancer
Fluorescence
Gelatinase
MMP
Optical imaging

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10.1117/12.810096

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Cheney, P. P., Fields, G. B., Achilefu, S., & Edwards, W. B. (2009). Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging. In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications Article 719011 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7190). https://doi.org/10.1117/12.810096

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abstract = "The prevalence of the gelatinases, MMP-2 and MMP-9, in many human tumors, including breast, colorectal, prostate and gastric cancer, make them an attractive target for molecular imaging. A self assembling homotrimeric triple helical peptide (THP), incorporating sequences from type V collagen with high specificity to MMP-2 and MMP-9, was previously developed. To investigate the viability of a THP for gelatinase imaging, we conjugated 5FAM to ε-amino groups of lysine flanking the hydrolysis site and subjected this substrate (THP-5FAM) to vitro analysis. The synthesis and in vitro results was presented.",

keywords = "Cancer, Fluorescence, Gelatinase, MMP, Optical imaging",

author = "Cheney, {Philip P.} and Fields, {Gregg B.} and Samuel Achilefu and Edwards, {W. Barry}",

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day = "15",

doi = "10.1117/12.810096",

language = "English",

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booktitle = "Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications",

note = "Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications ; Conference date: 26-01-2009 Through 29-01-2009",

}

Cheney, PP, Fields, GB, Achilefu, S & Edwards, WB 2009, Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging. in Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications., 719011, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications, San Jose, CA, United States, 01/26/09. https://doi.org/10.1117/12.810096

Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging. / Cheney, Philip P.; Fields, Gregg B.; Achilefu, Samuel et al.
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications. 2009. 719011 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7190).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging

AU - Cheney, Philip P.

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AU - Achilefu, Samuel

AU - Edwards, W. Barry

PY - 2009/6/15

Y1 - 2009/6/15

N2 - The prevalence of the gelatinases, MMP-2 and MMP-9, in many human tumors, including breast, colorectal, prostate and gastric cancer, make them an attractive target for molecular imaging. A self assembling homotrimeric triple helical peptide (THP), incorporating sequences from type V collagen with high specificity to MMP-2 and MMP-9, was previously developed. To investigate the viability of a THP for gelatinase imaging, we conjugated 5FAM to ε-amino groups of lysine flanking the hydrolysis site and subjected this substrate (THP-5FAM) to vitro analysis. The synthesis and in vitro results was presented.

AB - The prevalence of the gelatinases, MMP-2 and MMP-9, in many human tumors, including breast, colorectal, prostate and gastric cancer, make them an attractive target for molecular imaging. A self assembling homotrimeric triple helical peptide (THP), incorporating sequences from type V collagen with high specificity to MMP-2 and MMP-9, was previously developed. To investigate the viability of a THP for gelatinase imaging, we conjugated 5FAM to ε-amino groups of lysine flanking the hydrolysis site and subjected this substrate (THP-5FAM) to vitro analysis. The synthesis and in vitro results was presented.

KW - Cancer

KW - Fluorescence

KW - Gelatinase

KW - MMP

KW - Optical imaging

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T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications

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Y2 - 26 January 2009 through 29 January 2009

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Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging

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