TY - JOUR
T1 - Characterization of phosphatidylethanolamine as a lithiated adduct by triple quadrupole tandem mass spectrometry with electrospray ionization
AU - Hsu, Fong Fu
AU - Turk, John
PY - 2000
Y1 - 2000
N2 - Structural characterization of the glycerophosphoethanolamine (GPE) molecule as a lithiated adduct ion by collisionally activated dissociation (CAD) tandem mass spectrometry with electrospray ionization is described. Abundant fragment ions reflecting polar head group and fatty acid constituents were observed in the product ion spectrum of GPE, which permits an unambiguous structural determination, including the regiospecificity of fatty acyl substituents. The pathways leading to the formation of fragment ions are proposed. The suggested mechanisms are supported by the tandem mass spectra of various deuterated analogs and source CAD of GPE followed by CAD tandem mass spectrometry, Identification of GPE molecular species and specific GPE subclasses in a biological mixture by tandem mass spectrometry with various constant neutral loss scannings is also described. Copyright (C) 2000 John Wiley and Sons, Ltd.
AB - Structural characterization of the glycerophosphoethanolamine (GPE) molecule as a lithiated adduct ion by collisionally activated dissociation (CAD) tandem mass spectrometry with electrospray ionization is described. Abundant fragment ions reflecting polar head group and fatty acid constituents were observed in the product ion spectrum of GPE, which permits an unambiguous structural determination, including the regiospecificity of fatty acyl substituents. The pathways leading to the formation of fragment ions are proposed. The suggested mechanisms are supported by the tandem mass spectra of various deuterated analogs and source CAD of GPE followed by CAD tandem mass spectrometry, Identification of GPE molecular species and specific GPE subclasses in a biological mixture by tandem mass spectrometry with various constant neutral loss scannings is also described. Copyright (C) 2000 John Wiley and Sons, Ltd.
KW - Collisionally activated dissociation
KW - Electrospray ionization
KW - Phosphatidylethanolamine
KW - Phospholipid
KW - Tandem mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=0034181111&partnerID=8YFLogxK
U2 - 10.1002/(sici)1096-9888(200005)35:5<595::aid-jms965>3.0.co;2-d
DO - 10.1002/(sici)1096-9888(200005)35:5<595::aid-jms965>3.0.co;2-d
M3 - Article
C2 - 10800048
AN - SCOPUS:0034181111
SN - 1076-5174
VL - 35
SP - 595
EP - 606
JO - Journal of Mass Spectrometry
JF - Journal of Mass Spectrometry
IS - 5
ER -