Characterization of phosphate uptake in isolated chick intestinal cells.

R. Avioli; R. A. Miller; S. J. Birge

doi:10.1007/978-1-4615-9167-2_45

Characterization of phosphate uptake in isolated chick intestinal cells.

R. Avioli, R. A. Miller, S. J. Birge

Division of Nutritional Science & Obesity Medicine

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Abstract

A preparation of isolated intestinal cells has been developed which permits the incubation of these cells in culture for 90 min. with preservation of viability and responsiveness to vitamin D analogues added in vitro. Characterization of phosphate transport suggested the presence of two transport processes which differed in their affinity for phosphate, calcium stimulation, and pH dependence. Both processes were facilitated by sodium transport. This cell preparation promises to be a valuable experimental model for the elucidation of the action of vitamin D on phosphate transport.

Original language	English
Pages (from-to)	399-404
Number of pages	6
Journal	Advances in Experimental Medicine and Biology
Volume	128
DOIs	https://doi.org/10.1007/978-1-4615-9167-2_45
State	Published - 1980

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title = "Characterization of phosphate uptake in isolated chick intestinal cells.",

abstract = "A preparation of isolated intestinal cells has been developed which permits the incubation of these cells in culture for 90 min. with preservation of viability and responsiveness to vitamin D analogues added in vitro. Characterization of phosphate transport suggested the presence of two transport processes which differed in their affinity for phosphate, calcium stimulation, and pH dependence. Both processes were facilitated by sodium transport. This cell preparation promises to be a valuable experimental model for the elucidation of the action of vitamin D on phosphate transport.",

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AU - Miller, R. A.

AU - Birge, S. J.

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N2 - A preparation of isolated intestinal cells has been developed which permits the incubation of these cells in culture for 90 min. with preservation of viability and responsiveness to vitamin D analogues added in vitro. Characterization of phosphate transport suggested the presence of two transport processes which differed in their affinity for phosphate, calcium stimulation, and pH dependence. Both processes were facilitated by sodium transport. This cell preparation promises to be a valuable experimental model for the elucidation of the action of vitamin D on phosphate transport.

AB - A preparation of isolated intestinal cells has been developed which permits the incubation of these cells in culture for 90 min. with preservation of viability and responsiveness to vitamin D analogues added in vitro. Characterization of phosphate transport suggested the presence of two transport processes which differed in their affinity for phosphate, calcium stimulation, and pH dependence. Both processes were facilitated by sodium transport. This cell preparation promises to be a valuable experimental model for the elucidation of the action of vitamin D on phosphate transport.

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DO - 10.1007/978-1-4615-9167-2_45

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JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

ER -

Characterization of phosphate uptake in isolated chick intestinal cells.

Abstract

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