Characterization of MOPC 315 IgA oligosaccharide processing intermediates

Scot Hickman, Janice L. Theodorakis

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The structures of α1,2-mannose containing partially processed asparagine-linked oligosaccharides on the α-chain of MOPC 315 IgA were characterized using specific glycosidases and acetolysis. Man6GlcNAc2, a substrate for a Golgi α1,2-mannosidase, was found to be a single isomeric structure. Likewise, Man7-9GlcNAc2 were single isomers indicating an ordered sequence of removal of α1,2-linked mannose residues on this murine immunoglobulin heavy chain.

Original languageEnglish
Pages (from-to)586-593
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume128
Issue number2
DOIs
StatePublished - Apr 30 1985

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