Characterization of mice with a combined suppression of Ito and Ik,slow

Michael Brunner, Weinong Guo, Gary F. Mitchell, Peter D. Buckett, Jeanne M. Nerbonne, Gideon Koren

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Cardiac-specific expression of a truncated Kv1.1 polypeptide (Kv1DN) attenuates the slow inactivating outward K+ current (IK,slow), increases action potential duration (APD) and Q-T intervals, and induces spontaneous ventricular arrhythmias. Expression of the pore mutant of Kv4.2 (Kv4DN) eliminates the fast component of the transient outward current (Ito) and prolongs APDs and Q-T intervals markedly; however, no arrhythmias are seen in Kv4DN mice, suggesting that APD and Q-T prolongation are not per se proarrhythmic. To test this hypothesis, the Kv1DN and Kv4DN lines were crossbred to produce animals (Kv1/Kv4DN) expressing both transgenes in an identical genetic background. Whole cell voltage-clamp recordings from left ventricular apex cells confirmed that in Kv1/Kv4DN left ventricular apex cells, both components (fast and slow) of Ito and the 4-aminopyridine-sensitive component of IK,slow are eliminated, resulting in marked APD prolongation compared with wild-type, Kv1DN, or Kv4DN cells. Telemetric electrocardiogram monitoring (n = 10 mice/group) revealed a significant prolongation of Q-Tc and P-R intervals in Kv1/Kv4DN animals compared with Kv1DN or Kv4DN animals. Spontaneous arrhythmias were observed mainly in Kv1DN mice. Thus the attenuation of fast Ito in addition to IK,slow in Kv1/Kv4DN mice causes significant prolongation of APD and Q-T intervals and attenuation of spontaneous arrhythmias.

Original languageEnglish
Pages (from-to)H1201-H1209
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume281
Issue number3 50-3
DOIs
StatePublished - 2001

Keywords

  • Arrhythmia-basic studies
  • Cardiac voltage-gated potassium channels
  • Electrophysiology
  • Genetically altered mice

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