@article{ebf3f30838be4b4f8b3cf792b4c55f43,
title = "Characterization of Metabolic Patterns in Mouse Oocytes during Meiotic Maturation",
abstract = "Well-balanced and timed metabolism is essential for making a high-quality egg. However, the metabolic framework that supports oocyte development remains poorly understood. Here, we obtained the temporal metabolome profiles of mouse oocytes during in vivo maturation by isolating large number of cells at key stages. In parallel, quantitative proteomic analyses were conducted to bolster the metabolomic data, synergistically depicting the global metabolic patterns in oocytes. In particular, we discovered the metabolic features during meiotic maturation, such as the fall in polyunsaturated fatty acids (PUFAs) level and the active serine-glycine-one-carbon (SGOC) pathway. Using functional approaches, we further identified the key targets mediating the action of PUFA arachidonic acid (ARA) on meiotic maturation and demonstrated the control of epigenetic marks in maturing oocytes by SGOC network. Our data serve as a broad resource on the dynamics occurring in metabolome and proteome during oocyte maturation.",
keywords = "embryo, epigenetics, metabolism, oocyte, proteomics",
author = "Ling Li and Shuai Zhu and Wenjie Shu and Yueshuai Guo and Yusheng Guan and Juan Zeng and Haichao Wang and Longsen Han and Jiaqi Zhang and Xiaohui Liu and Chunling Li and Xiaojing Hou and Min Gao and Juan Ge and Chao Ren and Hao Zhang and Tim Schedl and Xuejiang Guo and Minjian Chen and Qiang Wang",
note = "Funding Information: This work was supported by National Key Scientific Research Projects (no. 2017YFC1001500 and 2018YFC1004000 to Q.W.; 2016YFA0503300 to X.G.), National Natural Science Foundation of China (no. 81925014 and 31771657 to Q.W.; no. 81872650 to M.C.), Science Foundation for Distinguished Young Scholars of Jiangsu Province (BK20180035 to Q.W.), and an NIH grant (R01GM100756 to T.S.). Q.W. M.C. and T.S. conceived the projects. M.C. and Y.G. contributed to the metabolomics profiling; Y.G. H.Z. and X.G. contributed to the proteomic profiling. L.L. S.Z. J.Z. H.W. and M.G. performed the mechanistic experiments. W.S. and C.R. contributed to the DNA methylome analysis. L.H. J.Z. X.H. X.L. C.L. and J.G. performed the oocyte/embryo collection and culture experiments. Q.W. wrote and T.S. revised the manuscript. The authors declare no competing interests. Funding Information: This work was supported by National Key Scientific Research Projects (no. 2017YFC1001500 and 2018YFC1004000 to Q.W.; 2016YFA0503300 to X.G.), National Natural Science Foundation of China (no. 81925014 and 31771657 to Q.W.; no. 81872650 to M.C.), Science Foundation for Distinguished Young Scholars of Jiangsu Province ( BK20180035 to Q.W.), and an NIH grant ( R01GM100756 to T.S.). Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = nov,
day = "5",
doi = "10.1016/j.molcel.2020.09.022",
language = "English",
volume = "80",
pages = "525--540.e9",
journal = "Molecular cell",
issn = "1097-2765",
number = "3",
}