Characterization of long terminal repeat sequences of HTLV-III

Bruno Starcich; L. E.E. Ratner; Steven F. Josephs; Takashi Okamoto; Robert C. Gallo; Flossie Wong-Staal

doi:10.1126/science.2981438

Characterization of long terminal repeat sequences of HTLV-III

Bruno Starcich, L. E.E. Ratner, Steven F. Josephs, Takashi Okamoto, Robert C. Gallo, Flossie Wong-Staal

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Abstract

The nucleotide sequence of the long terminal repeat sequence (LTR) of the human T-cell leukemia (lymphotropic) virus type III (HTLV-III) was determined. This virus is associated etiologically with the acquired immune deficiency syndrome. The LTR was found to be 634 base pairs in length with U3, R, and U5 regions of 453, 98, and 83 bp, respectively. The proviral DNA is flanked by a 7-base-pair direct repeat. The promoter and polyadenylation signals are situated 27 and 24 base pairs upstream from the respective transcriptional initiation and polyadenylation sites. The primer binding site is complementary to transfer RNA-lysine. The LTR of HTLV-III, like that of HTLV-I, showed a limited homology to enhancer-like sequences within two genes expressed specifically in T lymphocytes, T-cell growth factor, and γ-interferon. Structural comparisons revealed that the LTR of HTLV-III is distantly related to those of HTLV-I, HTLV-II, and bovine leukemia virus.

Original language	English
Pages (from-to)	538-540
Number of pages	3
Journal	Science
Volume	227
Issue number	4686
DOIs	https://doi.org/10.1126/science.2981438
State	Published - 1985

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@article{5301e23988674879af0ec5d0a8fdca74,

title = "Characterization of long terminal repeat sequences of HTLV-III",

abstract = "The nucleotide sequence of the long terminal repeat sequence (LTR) of the human T-cell leukemia (lymphotropic) virus type III (HTLV-III) was determined. This virus is associated etiologically with the acquired immune deficiency syndrome. The LTR was found to be 634 base pairs in length with U3, R, and U5 regions of 453, 98, and 83 bp, respectively. The proviral DNA is flanked by a 7-base-pair direct repeat. The promoter and polyadenylation signals are situated 27 and 24 base pairs upstream from the respective transcriptional initiation and polyadenylation sites. The primer binding site is complementary to transfer RNA-lysine. The LTR of HTLV-III, like that of HTLV-I, showed a limited homology to enhancer-like sequences within two genes expressed specifically in T lymphocytes, T-cell growth factor, and γ-interferon. Structural comparisons revealed that the LTR of HTLV-III is distantly related to those of HTLV-I, HTLV-II, and bovine leukemia virus.",

author = "Bruno Starcich and Ratner, {L. E.E.} and Josephs, {Steven F.} and Takashi Okamoto and Gallo, {Robert C.} and Flossie Wong-Staal",

year = "1985",

doi = "10.1126/science.2981438",

language = "English",

volume = "227",

pages = "538--540",

journal = "Science",

issn = "0036-8075",

number = "4686",

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TY - JOUR

T1 - Characterization of long terminal repeat sequences of HTLV-III

AU - Starcich, Bruno

AU - Ratner, L. E.E.

AU - Josephs, Steven F.

AU - Okamoto, Takashi

AU - Gallo, Robert C.

AU - Wong-Staal, Flossie

PY - 1985

Y1 - 1985

N2 - The nucleotide sequence of the long terminal repeat sequence (LTR) of the human T-cell leukemia (lymphotropic) virus type III (HTLV-III) was determined. This virus is associated etiologically with the acquired immune deficiency syndrome. The LTR was found to be 634 base pairs in length with U3, R, and U5 regions of 453, 98, and 83 bp, respectively. The proviral DNA is flanked by a 7-base-pair direct repeat. The promoter and polyadenylation signals are situated 27 and 24 base pairs upstream from the respective transcriptional initiation and polyadenylation sites. The primer binding site is complementary to transfer RNA-lysine. The LTR of HTLV-III, like that of HTLV-I, showed a limited homology to enhancer-like sequences within two genes expressed specifically in T lymphocytes, T-cell growth factor, and γ-interferon. Structural comparisons revealed that the LTR of HTLV-III is distantly related to those of HTLV-I, HTLV-II, and bovine leukemia virus.

AB - The nucleotide sequence of the long terminal repeat sequence (LTR) of the human T-cell leukemia (lymphotropic) virus type III (HTLV-III) was determined. This virus is associated etiologically with the acquired immune deficiency syndrome. The LTR was found to be 634 base pairs in length with U3, R, and U5 regions of 453, 98, and 83 bp, respectively. The proviral DNA is flanked by a 7-base-pair direct repeat. The promoter and polyadenylation signals are situated 27 and 24 base pairs upstream from the respective transcriptional initiation and polyadenylation sites. The primer binding site is complementary to transfer RNA-lysine. The LTR of HTLV-III, like that of HTLV-I, showed a limited homology to enhancer-like sequences within two genes expressed specifically in T lymphocytes, T-cell growth factor, and γ-interferon. Structural comparisons revealed that the LTR of HTLV-III is distantly related to those of HTLV-I, HTLV-II, and bovine leukemia virus.

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U2 - 10.1126/science.2981438

DO - 10.1126/science.2981438

M3 - Article

C2 - 2981438

AN - SCOPUS:0022001452

SN - 0036-8075

VL - 227

SP - 538

EP - 540

JO - Science

JF - Science

IS - 4686

ER -

Characterization of long terminal repeat sequences of HTLV-III

Abstract

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