TY - JOUR
T1 - Characterization of Long-Chain Fatty Acid as N-(4-Aminomethylphenyl) Pyridinium Derivative by MALDI LIFT-TOF/TOF Mass Spectrometry
AU - Frankfater, Cheryl
AU - Jiang, Xuntian
AU - Hsu, Fong Fu
N1 - Funding Information:
This research was supported by US Public Health Service Grants P41GM103422, P30DK020579, R01AI130454, R21AI128427, and R21HL120760.
Publisher Copyright:
© 2018, American Society for Mass Spectrometry.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Charge remote fragmentation (CRF) elimination of C n H 2n+2 residues along the aliphatic tail of long chain fatty acid is hall mark of keV high-energy CID fragmentation process. It is an important fragmentation pathway leading to structural characterization of biomolecules by CID tandem mass spectrometry. In this report, we describe MALDI LIFT TOF-TOF mass spectrometric approach to study a wide variety of fatty acids (FAs), which were derivatized to N-(4-aminomethylphenyl) pyridinium (AMPP) derivative, and desorbed as M + ions by laser with or without matrix. The high-energy MALDI LIFT TOF-TOF mass spectra of FA-AMPP contain fragment ions mainly deriving from CRF cleavages of C n H 2n+2 residues, as expected. These ions together with ions from specific cleavages of the bond(s) involving the functional group within the molecule provide more complete structural identification than those produced by low-energy CID/HCD using a linear ion-trap instrument. However, this LIFT TOF-TOF mass spectrometric approach inherits low sensitivity, a typical feature of high-energy CID tandem mass spectrometry. Because of the lack of unit mass precursor ion selection with sufficient sensitivity of the current LIFT TOF-TOF technology, product ion spectra from same chain length fatty acids with difference in one or two double bonds in a mixture are not distinguishable.
AB - Charge remote fragmentation (CRF) elimination of C n H 2n+2 residues along the aliphatic tail of long chain fatty acid is hall mark of keV high-energy CID fragmentation process. It is an important fragmentation pathway leading to structural characterization of biomolecules by CID tandem mass spectrometry. In this report, we describe MALDI LIFT TOF-TOF mass spectrometric approach to study a wide variety of fatty acids (FAs), which were derivatized to N-(4-aminomethylphenyl) pyridinium (AMPP) derivative, and desorbed as M + ions by laser with or without matrix. The high-energy MALDI LIFT TOF-TOF mass spectra of FA-AMPP contain fragment ions mainly deriving from CRF cleavages of C n H 2n+2 residues, as expected. These ions together with ions from specific cleavages of the bond(s) involving the functional group within the molecule provide more complete structural identification than those produced by low-energy CID/HCD using a linear ion-trap instrument. However, this LIFT TOF-TOF mass spectrometric approach inherits low sensitivity, a typical feature of high-energy CID tandem mass spectrometry. Because of the lack of unit mass precursor ion selection with sufficient sensitivity of the current LIFT TOF-TOF technology, product ion spectra from same chain length fatty acids with difference in one or two double bonds in a mixture are not distinguishable.
KW - Branched fatty acids
KW - Charge switch derivatization
KW - HCD
KW - High-energy CID
KW - Linear ion-trap
KW - Lipidomics
KW - Microbial lipids
UR - http://www.scopus.com/inward/record.url?scp=85050080411&partnerID=8YFLogxK
U2 - 10.1007/s13361-018-1993-z
DO - 10.1007/s13361-018-1993-z
M3 - Article
C2 - 29855891
AN - SCOPUS:85050080411
SN - 1044-0305
VL - 29
SP - 1688
EP - 1699
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
IS - 8
ER -