Characterization of insoluble elastin from copper-deficient pigs. Its usefulness in elastin sequence studies

Robert P. Mecham, Judith A. Foster

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Insoluble elastin from copper-deficient animals has an amino acid composition intermediate between mature elastin and salt-soluble elastin (a higher lysine content and correspondingly low number of cross-links relative to the normal protein) and is solubilized by successive treatment with trypsin and chymotrypsin at 4 and 37°C. Small amounts of B3H4 (11 mg-2 g of elastin) reduced allysine, allysine aldol, dehydronorleucine, and dehydromerodesmosine in insoluble elastin from copper-deficient pig aorta. In contrast, desmosine and isodesmosine were reduced only when a large excess of reductant (400 mg borohydride) was included in the reaction mixture. Reduction studies indicated that lysinonorleucine and merodesmosine were present in their dehydro forms to a greater extent in copper-deficient pig elastin than in normal elastin. After reduction with borohydride approximately 35% of the reduced form of the insoluble elastin remained insoluble after digestion with trypsin and chymotrypsin. A peptide containing the aldehyde oxidation product of lysine (allysine) and demonstrating an enrichment in glutamic acid was purified from the reduced form of copper-deficient pig elastin and partially sequenced. Its sequence (Gly-Ala-Glu-allysine-(Glu)...) and amino acid composition suggest: (1) clustering of glutamic acid residues in the elastin molecule, and (2) that allysine residues are not restricted to the alanine-enriched sites described for other elastin crosslinks. Insoluble elastin from copper-deficient animals promises to be a useful tool for elastin sequence studies.

Original languageEnglish
Pages (from-to)147-158
Number of pages12
JournalBBA - Protein Structure
Volume577
Issue number1
DOIs
StatePublished - Mar 27 1979

Keywords

  • Allysine
  • Copper deficiency
  • Cross-link
  • Elastin sequence

Fingerprint Dive into the research topics of 'Characterization of insoluble elastin from copper-deficient pigs. Its usefulness in elastin sequence studies'. Together they form a unique fingerprint.

Cite this