Characterization of Distal and Proximal Alternative Promoters of the Human ApoA-I Gene

D. A. Mogilenko, V. S. Shavva, E. B. Dizhe, S. V. Orlov

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Abstract: Human apolipoprotein A-I (ApoA-I) is a major structural and functional protein component of high-density lipoprotein (HDL). ApoA-I constitutes ~75% of the protein content of HDL. The main sites of ApoA-I synthesis in humans are the liver and the small intestine. The mechanisms that govern tissue-specific apoA-I transcription in tissues and organs other than the liver and the small intestine are poorly understood. It is known that the human apoA-I has two additional promoters, the proximal and the distal one. In this work these two alternative apoA-I promoters are characterized, their transcription start sites are mapped and their competition for apoA-I transcription is demonstrated; the role of the alternative promoters in apoA-I expression in human cells and tissues other than hepatocytes and enterocytes is discussed.

Original languageEnglish
Pages (from-to)427-437
Number of pages11
JournalMolecular Biology
Volume53
Issue number3
DOIs
StatePublished - May 1 2019

Keywords

  • alternative promoter
  • apolipoprotein A-I
  • human hepatoma cells HepG2
  • promoter competition
  • transcription start site

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