TY - JOUR
T1 - Characterization of colorectal cancer development in Apcmin/+mice
AU - Nalbantoglu, Ilke
AU - Blanc, Valerie
AU - Davidson, Nicholas O.
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2016.
PY - 2016
Y1 - 2016
N2 - The Apcmin/+mouse provides an excellent experimental model for studying genetic, environmental, and therapeutic aspects of intestinal neoplasia in humans. In this chapter, we will describe techniques for studying colon cancer development in Apcmin/+mice on C57BL/6J (B6) background, focusing on the roles of environmental modifiers, including Dextran Sulfate Sodium (DSS), high fat diet, and bile acid supplementation in the context of experimental colorectal cancer. This chapter also includes protocols describing extraction and purification of DSS-contaminated RNA, as well as sampling, harvesting, and tissue processing. The common pathologic lesions encountered in these animals are described in detail.
AB - The Apcmin/+mouse provides an excellent experimental model for studying genetic, environmental, and therapeutic aspects of intestinal neoplasia in humans. In this chapter, we will describe techniques for studying colon cancer development in Apcmin/+mice on C57BL/6J (B6) background, focusing on the roles of environmental modifiers, including Dextran Sulfate Sodium (DSS), high fat diet, and bile acid supplementation in the context of experimental colorectal cancer. This chapter also includes protocols describing extraction and purification of DSS-contaminated RNA, as well as sampling, harvesting, and tissue processing. The common pathologic lesions encountered in these animals are described in detail.
KW - Adenoma
KW - Apc
KW - Bile acids
KW - Colorectal cancer
KW - Dextran sulfate sodium
KW - High fat
UR - http://www.scopus.com/inward/record.url?scp=84990068255&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3603-8_27
DO - 10.1007/978-1-4939-3603-8_27
M3 - Article
C2 - 27246043
AN - SCOPUS:84990068255
SN - 1064-3745
VL - 1422
SP - 309
EP - 327
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -