TY - JOUR
T1 - Characterization of cell membrane extensions and studying their roles in cancer cell adhesion dynamics
AU - Brown, Taylor C.
AU - Cheng, Joyce
AU - Korah, Reju
AU - Carling, Tobias
N1 - Publisher Copyright:
© 2018 Journal of Visualized Experiments.
PY - 2018/3/14
Y1 - 2018/3/14
N2 - The cell membrane's extension repertoire modulates various malignant behaviors of cancer cells, including their adhesive and migratory potentials. The ability to accurately classify and quantify cell extensions and measure the effect on a cell's adhesive capacity is critical to determining how cell-signaling events impact cancer cell behavior and aggressiveness. Here, we describe the in vitro design and use of a cell extension quantification method in conjunction with an adhesion capacity assay in an established in vitro model for adrenocortical carcinoma (ACC). Specifically, we test the effects of DKK3, a putative tumor suppressor and a pro-differentiation factor, on the membrane extension phenotype of the ACC cell line, SW-13. We propose these assays to provide relatively simple, reliable, and easily interpretable metrics to measures these characteristics under various experimental conditions.
AB - The cell membrane's extension repertoire modulates various malignant behaviors of cancer cells, including their adhesive and migratory potentials. The ability to accurately classify and quantify cell extensions and measure the effect on a cell's adhesive capacity is critical to determining how cell-signaling events impact cancer cell behavior and aggressiveness. Here, we describe the in vitro design and use of a cell extension quantification method in conjunction with an adhesion capacity assay in an established in vitro model for adrenocortical carcinoma (ACC). Specifically, we test the effects of DKK3, a putative tumor suppressor and a pro-differentiation factor, on the membrane extension phenotype of the ACC cell line, SW-13. We propose these assays to provide relatively simple, reliable, and easily interpretable metrics to measures these characteristics under various experimental conditions.
KW - Adhesion
KW - Adrenocortical cancer
KW - Cell membrane extensions
KW - DKK3
KW - Filopodia
KW - Lamellipodia
KW - Lobopodia
UR - http://www.scopus.com/inward/record.url?scp=85044665963&partnerID=8YFLogxK
U2 - 10.3791/56560
DO - 10.3791/56560
M3 - Article
C2 - 29630043
AN - SCOPUS:85044665963
SN - 1940-087X
VL - 2018
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
IS - 133
M1 - e56560
ER -