Characterization of cell membrane extensions and studying their roles in cancer cell adhesion dynamics

Taylor C. Brown, Joyce Cheng, Reju Korah, Tobias Carling

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The cell membrane's extension repertoire modulates various malignant behaviors of cancer cells, including their adhesive and migratory potentials. The ability to accurately classify and quantify cell extensions and measure the effect on a cell's adhesive capacity is critical to determining how cell-signaling events impact cancer cell behavior and aggressiveness. Here, we describe the in vitro design and use of a cell extension quantification method in conjunction with an adhesion capacity assay in an established in vitro model for adrenocortical carcinoma (ACC). Specifically, we test the effects of DKK3, a putative tumor suppressor and a pro-differentiation factor, on the membrane extension phenotype of the ACC cell line, SW-13. We propose these assays to provide relatively simple, reliable, and easily interpretable metrics to measures these characteristics under various experimental conditions.

Original languageEnglish
Article numbere56560
JournalJournal of Visualized Experiments
Issue number133
StatePublished - Mar 14 2018


  • Adhesion
  • Adrenocortical cancer
  • Cell membrane extensions
  • DKK3
  • Filopodia
  • Lamellipodia
  • Lobopodia


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