TY - JOUR
T1 - Characterization of antibodies for various G-protein β and γ subunits
AU - Pronin, Alexey N.
AU - Gautam, Narasimhan
N1 - Funding Information:
N.G. is an Established Investigator of the American Heart Association. This work was supported by grants from the National Institutes of Health and Monsanto-Searle.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - Antibodies to the β and γ subunits of G proteins have been invaluable in the investigations that have begun to dissect the structure and function of these subunits. The cDNAs for four β subunit types and for several γ subunits have now been characterized. The most useful antisera are those raised against peptides specific to the amino acid sequence encoded by cDNAs for different β and γ subunit types. Depending on the region of the protein chosen for synthesizing a corresponding peptide, these antibodies are capable of reacting with a particular subunit type specifically or with several different subunits. Of an immunoblot of a mixture of different subunit types, specific peptide antisera can distinguish among different members of the β and γ subunit families of proteins. Although the antisera are raised against a peptide corresponding to a relatively small portion of the protein, several of the antisera against the G-protein β and γ subunits are capable of immunoprecipitating the βγ complex. They can also be used for immunocytochemical localization of the β or γ subunits present in transient transfectants.
AB - Antibodies to the β and γ subunits of G proteins have been invaluable in the investigations that have begun to dissect the structure and function of these subunits. The cDNAs for four β subunit types and for several γ subunits have now been characterized. The most useful antisera are those raised against peptides specific to the amino acid sequence encoded by cDNAs for different β and γ subunit types. Depending on the region of the protein chosen for synthesizing a corresponding peptide, these antibodies are capable of reacting with a particular subunit type specifically or with several different subunits. Of an immunoblot of a mixture of different subunit types, specific peptide antisera can distinguish among different members of the β and γ subunit families of proteins. Although the antisera are raised against a peptide corresponding to a relatively small portion of the protein, several of the antisera against the G-protein β and γ subunits are capable of immunoprecipitating the βγ complex. They can also be used for immunocytochemical localization of the β or γ subunits present in transient transfectants.
UR - http://www.scopus.com/inward/record.url?scp=0028104038&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(94)37085-0
DO - 10.1016/S0076-6879(94)37085-0
M3 - Article
C2 - 7935022
AN - SCOPUS:0028104038
SN - 0076-6879
VL - 237
SP - 482
EP - 498
JO - Methods in enzymology
JF - Methods in enzymology
IS - C
ER -