Abstract
We have cloned and characterized a cDNA encoding a putative ETS transcription factor, designated Cf-ets. The Cf-ets encodes a 406 amino acid protein containing a conserved ETS domain and a Pointed domain. Phylogenetic analysis revealed that Cf-ets belongs to the ESE group of ETS transcription factor family. Real-time PCR analysis of Cf-ets expression in adult sea scallop tissues revealed that Cf-ets was expressed mainly in gill and hemocytes, in a constitutive manner. Cf-ets mRNA level in hemocytes increased drastically after microbial challenge indicated its indispensable role in the anti-infection process. Simultaneously, the circulating hemocyte number decreased. In mammals, most ETS transcription factors play indispensable roles in blood cell differentiation and linage commitment during hematopoisis. Cf-ets is therefore likely to be a potential biomarker for hematopoiesis studies in scallops.
Original language | English |
---|---|
Pages (from-to) | 953-958 |
Number of pages | 6 |
Journal | Developmental and Comparative Immunology |
Volume | 33 |
Issue number | 9 |
DOIs | |
State | Published - Sep 2009 |
Keywords
- Cloning
- Hematopoiesis
- Hemocyte count
- Immune challenge
- Mollusk
- Phylogeny
- Real-time PCR
- Suppressive subtractive hybridization