Abstract
An improved procedure has been developed to prepare RCOOPO2-Ado (R = C6H13 and C15H31), the intermediate in the enzymatic synthesis of acyl-CoA's. The product has been characterized by spectral methods which, in turn, were used to define the hydrolysis rates of RCO-AMP. When R is a fatty acyl group, the hydrolysis rate is 10-fold slower than when R is aminoacyl. In both cases, the hydrolysis rate is enhanced by Mg2+. We speculate that the rate acceleration is due to intramolecular participation of the second carbonyl group in the aminoacyl residue.
Original language | English |
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Pages (from-to) | 8661-8667 |
Number of pages | 7 |
Journal | Journal of Organic Chemistry |
Volume | 63 |
Issue number | 24 |
DOIs | |
State | Published - Nov 27 1998 |