@article{09aa7951ab9c47478dfb18bbc1378500,
title = "Characterization of a rat kidney thromboxane A2 receptor: High affinity for the agonist ligand I-BOP",
abstract = "We have cloned a rat kidney thromboxane A2 receptor (TP) cDNA. This receptor was shown to be functional in that the thromboxane A2 mimetics, U46619 and I-BOP, elicited calcium transients in Xenopus oocytes and HEK293 cells expressing the TP receptor, respectively. Comparison of the affinities of the rat and human TP sites for the agonist radioligand [125I]BOP showed that the rat TP site has about a ten-fold higher affinity for this drug (K(D) = 0.5 vs. 4.4 nM) while the affinities of the two sites for other compounds (U46619, I-PTH-OH) were the same. Our results are significant in that they identify a cloned TP as having a picomolar affinity for [125I]BOP.",
keywords = "HEK293 cells, I-BOP, molecular cloning, oocyte expression (Xenopus laevis), radioligand binding, thromboxane A receptor",
author = "D'Angelo, {Drew D.} and Takayuki Terasawa and Carlisle, {Steven J.} and Dorn, {Gerald W.} and Lynch, {Kevin R.}",
note = "Funding Information: The authors acknowledge gratefully the gifts of the rat M, muscarinic acetylcholine receptor cDNA from Dr. Tom I. Bonner and the I-BOP precursor and I-PTA-OH from Dr. Perry Halushka. Support: D. D{\textquoteright}Angelo was supported during these studies by a Fellowship from the Ohio Affiliate of the American Heart Association while T. Terasawa was supported by a Fellowship from G.D. Searle, Inc. This work was supported in part by grants from the National Institutes of Health (HL19242 to KRL; HL49267 to GWD) and a Merit Review grant from the Veterans Administration (GWD). G.W. Dorn is an Established Investigator of the American Heart Association.",
year = "1996",
month = oct,
doi = "10.1016/S0090-6980(96)00091-3",
language = "English",
volume = "52",
pages = "303--316",
journal = "Prostaglandins",
issn = "0090-6980",
number = "4",
}