Characterization of a porcine intestinal epithelial cell line for influenza virus production

Zhi Sun, Victor C. Huber, Kara Mccormick, Radhey S. Kaushik, Adrianus C.M. Boon, Longchao Zhu, Ben Hause, Richard J. Webby, Ying Fang

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

We have developed a porcine intestine epithelial cell line, designated SD-PJEC for the propagation of influenza viruses. The SD-PJEC cell line is a subclone of the IPEC-J2 cell line, which was originally derived from newborn piglet jejunum. Our results demonstrate that SD-PJEC is a cell line of epithelial origin that preferentially expresses receptors of oligosaccharides with Sia2-6Gal modification. This cell line is permissive to infection with human and swine influenza A viruses and some avian influenza viruses, but poorly support the growth of human-origin influenza B viruses. Propagation of swine-origin influenza viruses in these cells results in a rapid growth rate within the first 24 h post-infection and the titres ranged from 4 to 8 log 10 TCID 50 ml -1. The SDPJEC cell line was further tested as a potential alternative cell line to Madin-Darby canine kidney (MDCK) cells in conjunction with 293T cells for rescue of swine-origin influenza viruses using the reverse genetics system. The recombinant viruses A/swine/North Carolina/18161/02 (H1N1) and A/swine/Texas/4199-2/98 (H3N2) were rescued with virus titres of 7 and 8.25 log 10 TCID 50 ml -1, respectively. The availability of this swine-specific cell line represents a more relevant substrate for studies and growth of swine-origin influenza viruses.

Original languageEnglish
Pages (from-to)2008-2016
Number of pages9
JournalJournal of General Virology
Volume93
Issue numberPART 9
DOIs
StatePublished - Sep 2012

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