Characterization of a murine model of recurrent herpes simplex viral keratitis induced by ultraviolet B radiation

K. A. Laycock, S. F. Lee, R. H. Brady, J. S. Pepose

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Abstract

The authors characterized a murine model of herpes simplex virus (HSV) reactivation in which recurrent herpetic keratitis was obtained in up to 80% of animals. Five weeks after ganglionic latency was established in National Institutes of Health inbred mice after corneal inoculation, HSV type 1 (HSV- 1) was reactivated by irradiating the previously inoculated eye with ultraviolet (UV) light. Comparison of different UV wavelengths showed UVB to be optimal for reactivation, with peak viral recurrence being induced by a total exposure of approximately 250 mJ/cm2. Reactivated infectious virus generally began to appear in trigeminal ganglia 2 days postirradiation and was subsequently detectable in the cornea by both corneal swabbing and immunostaining for viral antigens. Two consecutive outbreaks of viral recurrence at the ocular surface were induced in selected animals by serial exposure to UVB. Advantages of this model over other models of recurrent keratitis are discussed.

Original languageEnglish
Pages (from-to)2741-2746
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume32
Issue number10
StatePublished - Jan 1 1991

Keywords

  • herpes simplex
  • keratitis
  • ultraviolet animal models
  • viral recurrence

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