TY - JOUR
T1 - Characterization of a microvillous membrane preparation from human placental syncytiotrophoblast
T2 - a morphologic, biochemical, and physiologic study
AU - Smith, C. H.
AU - Nelson, D. M.
AU - King, B. F.
AU - Donohue, T. M.
AU - Ruzycki, S.
AU - Kelley, L. K.
N1 - Funding Information:
Financial support for this investigation was provided by Grant No. CRBS-309 from the National Foundation-March of Dimes (C. H. S.) and funds from National Institutes of Health Traineeship GM02016 (D. M. N.) and Grants HD07562 (C. H. S.), HD06734 (B. F. K.), and HD10344 (to Dr. Allen C. F.n.denl SinMmrt to Dr. Kinvwas also brovicled II'V Research c~;;e;,D~v;foJ;nent A~arlK04 HD00067. ' Abbreviations used in the text are as follows: EBSS, Earle's balanced salt solution; PBS, phosphate-buffered saline; PAP, peroxidase-antiperoxidase; SDS, sodium dodecyl sulfate; TCA, trichloroacetic acid; KRP, Krebs-Ringer phosphate buffer; Tris, Tris (hydroxymethyl) aminomethane.
PY - 1977
Y1 - 1977
N2 - The syncytiotrophoblast microvillous membrane must play a vital role in many essential functions of the placenta. In order to better understand the functional characteristics of this membrane, the authors have investigated an isolated membrane preparation by a variety of techniques. Electron microscopic observations showed membranous structures similar to microvilli of intact placental villi in size, shape, and microfilamentous content. Similarities in colloidal iron staining and transferrin localization were also shown. The preparation was enriched in enzymes characteristic of surface membranes and diminished in enzymes characteristic of intracellular organelles. Sialic acid content was also increased. SDS gel electrophoresis revealed the presence of a 45,000 molecular weight band, which may be actin. The preparation transported serine, glycine, and α aminoisobutyric acid by a temperature dependent, saturable process.
AB - The syncytiotrophoblast microvillous membrane must play a vital role in many essential functions of the placenta. In order to better understand the functional characteristics of this membrane, the authors have investigated an isolated membrane preparation by a variety of techniques. Electron microscopic observations showed membranous structures similar to microvilli of intact placental villi in size, shape, and microfilamentous content. Similarities in colloidal iron staining and transferrin localization were also shown. The preparation was enriched in enzymes characteristic of surface membranes and diminished in enzymes characteristic of intracellular organelles. Sialic acid content was also increased. SDS gel electrophoresis revealed the presence of a 45,000 molecular weight band, which may be actin. The preparation transported serine, glycine, and α aminoisobutyric acid by a temperature dependent, saturable process.
UR - http://www.scopus.com/inward/record.url?scp=0017713832&partnerID=8YFLogxK
U2 - 10.1016/0002-9378(77)90686-X
DO - 10.1016/0002-9378(77)90686-X
M3 - Article
C2 - 855869
AN - SCOPUS:0017713832
VL - 128
SP - 190
EP - 196
JO - Unknown Journal
JF - Unknown Journal
IS - 2
ER -