Characterization of a (Ca2+ +Mg2+)-ATPase system in the osteoblast plasma membrane

V. Shen, K. Hruska, L. V. Avioli

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Abstract

A high affinity, calmodulin-sensitive (Ca2 + Mg2)-ATPase was demonstrated in the plasma membrane preparation of three different osteosarcoma cell lines previously demonstrated to respond to parathyroid hormone with an increase in cytosolic calcium and a decrease in pH. The maximal velocity of the ezyme activity in the membrane preparations ranged from 0.83 to 2.42 nmol Pi released per min per mg protein with half-saturation constants of 26 nM of free Ca. The enzyme activity was not affected by Na+, K+, ouabain and azide, and exhibited an absolute requirement for Mg2+ ions. These results suggest a possible role for a membrane Ca2 + Mg2+-ATPase in initiating and perpetuating the ionic control of osteoblastic function.

Original languageEnglish
Pages (from-to)325-329
Number of pages5
JournalBone
Volume9
Issue number5
DOIs
StatePublished - 1988

Keywords

  • (Ca + Mg)-ATPase
  • Plasma

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