TY - JOUR
T1 - Characterization and quantitation of peptide-MHC complexes produced from hen egg lysozyme using a monoclonal antibody
AU - Dadaglio, Gilles
AU - Nelson, Christopher A.
AU - Deck, M. Brian
AU - Petzold, Shirley J.
AU - Unanue, Emil R.
PY - 1997/6
Y1 - 1997/6
N2 - Here we describe generation of Aw3.18, a monoclonal antibody that recognizes peptide residues 48-62 of hen egg lysozyme (HEL) bound to the MHC class II molecule I-A(k). Epitope mapping revealed that Aw3.18 detects a change in the solvent-exposed surface of this peptide-MHC complex upon substitution of the peptide side chain at position P1. Furthermore, Aw3.18 blocked recognition by some, but not all, of the HEL 48-62-reactive T cell hybridomas tested, suggesting a heterogeneity in the T cell response toward this complex. Finally, using Aw3.18, it was possible to determine the fraction of I-A(k) molecules loaded with 48-62 peptide after culture of an antigen-presenting cell in medium containing HEL.
AB - Here we describe generation of Aw3.18, a monoclonal antibody that recognizes peptide residues 48-62 of hen egg lysozyme (HEL) bound to the MHC class II molecule I-A(k). Epitope mapping revealed that Aw3.18 detects a change in the solvent-exposed surface of this peptide-MHC complex upon substitution of the peptide side chain at position P1. Furthermore, Aw3.18 blocked recognition by some, but not all, of the HEL 48-62-reactive T cell hybridomas tested, suggesting a heterogeneity in the T cell response toward this complex. Finally, using Aw3.18, it was possible to determine the fraction of I-A(k) molecules loaded with 48-62 peptide after culture of an antigen-presenting cell in medium containing HEL.
UR - http://www.scopus.com/inward/record.url?scp=0030745938&partnerID=8YFLogxK
U2 - 10.1016/S1074-7613(00)80448-3
DO - 10.1016/S1074-7613(00)80448-3
M3 - Article
C2 - 9208845
AN - SCOPUS:0030745938
SN - 1074-7613
VL - 6
SP - 727
EP - 738
JO - Immunity
JF - Immunity
IS - 6
ER -