Characterization and quantitation of peptide-MHC complexes produced from hen egg lysozyme using a monoclonal antibody

Gilles Dadaglio, Christopher A. Nelson, M. Brian Deck, Shirley J. Petzold, Emil R. Unanue

Research output: Contribution to journalArticlepeer-review

137 Scopus citations

Abstract

Here we describe generation of Aw3.18, a monoclonal antibody that recognizes peptide residues 48-62 of hen egg lysozyme (HEL) bound to the MHC class II molecule I-A(k). Epitope mapping revealed that Aw3.18 detects a change in the solvent-exposed surface of this peptide-MHC complex upon substitution of the peptide side chain at position P1. Furthermore, Aw3.18 blocked recognition by some, but not all, of the HEL 48-62-reactive T cell hybridomas tested, suggesting a heterogeneity in the T cell response toward this complex. Finally, using Aw3.18, it was possible to determine the fraction of I-A(k) molecules loaded with 48-62 peptide after culture of an antigen-presenting cell in medium containing HEL.

Original languageEnglish
Pages (from-to)727-738
Number of pages12
JournalImmunity
Volume6
Issue number6
DOIs
StatePublished - Jun 1997

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