Characteristics of [3H]prazosin binding to guinea pig brain α-adrenergic receptors: effects of estradiol and progesterone

Bruce Nock, Harvey H. Feder

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

[3H]Prazosin was found to bind to sites on guinea pig brain membranes with α1-adrenergic receptor characteristics. Treatment of ovariectomized guinea pigs with estradiol benzoate (EB) or EB followed by progesterone (P) did not affect [3H]prazosin binding to membranes from hypothalamus, preoptic area, amygdala or cerebral cortex. When added to the incubation mixture of the assay, estradiol, P, and other steroids decreased [3H]prazosin binding but only at high concentrations. These results do not support the idea that estrogen and progestin influence reproductive physiology through effects on brain α1-receptors, although limitations of the methodology employed do not completely rule out this possibility.

Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalBrain Research
Volume262
Issue number1
DOIs
StatePublished - Feb 28 1983
Externally publishedYes

Keywords

  • [H]prazosin binding
  • estradiol
  • guinea pig brain
  • prazosin
  • progesterone
  • steroid hormone
  • α-adrenergic receptor

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