@article{da25adf1eada4dbc977b74af43c4ca67,
title = "Characteristics of [3H]prazosin binding to guinea pig brain α-adrenergic receptors: effects of estradiol and progesterone",
abstract = "[3H]Prazosin was found to bind to sites on guinea pig brain membranes with α1-adrenergic receptor characteristics. Treatment of ovariectomized guinea pigs with estradiol benzoate (EB) or EB followed by progesterone (P) did not affect [3H]prazosin binding to membranes from hypothalamus, preoptic area, amygdala or cerebral cortex. When added to the incubation mixture of the assay, estradiol, P, and other steroids decreased [3H]prazosin binding but only at high concentrations. These results do not support the idea that estrogen and progestin influence reproductive physiology through effects on brain α1-receptors, although limitations of the methodology employed do not completely rule out this possibility.",
keywords = "[H]prazosin binding, estradiol, guinea pig brain, prazosin, progesterone, steroid hormone, α-adrenergic receptor",
author = "Bruce Nock and Feder, {Harvey H.}",
note = "Funding Information: We thank Natalie Marangi and Allan Johnson for their technical assistance, and Winona Cunningham for typing. We also thank Lynn O'Connor for helpful discussion concerning this research. Phenoxybenzamine, clonidine, phentolamine and unlabeled prazosin were generously provided by A. Tanaka of Smith, Kline and French Labs., Dr. S. B. Garbus of Boehringer Ingelheim, C. A. Brownley, Jr. of CIBA pharmaceuticals, and E. M. Weiss of Pfizer, respectively. Supported by NIH Research Grant HD-04467, NIMH Career Development Award MH-29006 and a grant from the Busch Foundation, Rutgers University (all to H.H.F.). B.N. was supported by Training Grant NIMH 15145-03. Contribution number 183 of the Institute of Animal Behavior.",
year = "1983",
month = feb,
day = "28",
doi = "10.1016/0006-8993(83)90482-1",
language = "English",
volume = "262",
pages = "163--167",
journal = "Brain Research",
issn = "0006-8993",
number = "1",
}