Characteristics of concatemeric GABA A receptors containing α4/δ subunits expressed in Xenopus oocytes

Hong Jin Shu, John Bracamontes, Amanda Taylor, Kyle Wu, Megan M. Eaton, Gustav Akk, Brad Manion, Alex S. Evers, Kathiresan Krishnan, Douglas F. Covey, Charles F. Zorumski, Joe Henry Steinbach, Steven Mennerick

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

BACKGROUND AND PURPOSE GABA A receptors mediate both synaptic and extrasynaptic actions of GABA. In several neuronal populations, α4 and δ subunits are key components of extrasynaptic GABA A receptors that strongly influence neuronal excitability and could mediate the effects of neuroactive agents including neurosteroids and ethanol. However, these receptors can be difficult to study in native cells and recombinant δ subunits can be difficult to express in heterologous systems. EXPERIMENTAL APPROACH We engineered concatemeric (fused) subunits to ensure δ and α4 subunit expression. We tested the pharmacology of the concatemeric receptors, compared with a common synaptic-like receptor subunit combination (α1 +β2 +γ2L), and with free-subunit α4/δ receptors, expressed in Xenopus oocytes. KEY RESULTS δ-β2 -α4 +β2-α4 cRNA co-injected into Xenopus oocytes resulted in GABA-gated currents with the expected pharmacological properties of α4/δ-containing receptors. Criteria included sensitivity to agonists of different efficacy, sensitivity to the allosteric activator pentobarbital, and modulation of agonist responses by DS2 (4-chloro-N-[2-(2-thienyl)imidazo[1,2-a]pyridine-3-yl benzamide; a δ-selective positive modulator), furosemide, and Zn 2+. We used the concatemers to examine neurosteroid sensitivity of extrasynaptic-like, δ-containing receptors. We found no qualitative differences between extrasynaptic-like receptors and synaptic-like receptors in the actions of either negative or positive neurosteroid modulators of receptor function. Quantitative differences were explained by the partial agonist effects of the natural agonist GABA and by a mildly increased sensitivity to low steroid concentrations. CONCLUSIONS AND IMPLICATIONS The neurosteroid structure-activity profile for α4/δ-containing extrasynaptic receptors is unlikely to differ from that of synaptic-like receptors such as α1/β2/γ2- containing receptors.

Original languageEnglish
Pages (from-to)2228-2243
Number of pages16
JournalBritish Journal of Pharmacology
Volume165
Issue number7
DOIs
StatePublished - Apr 2012

Keywords

  • GABA
  • acetylcholine
  • ligand-gated channels
  • molecular pharmacology
  • neuropharmacology
  • steroids/neurosteroids

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