@inbook{df0be96c86ae46cda53f226d9fc18872,
title = "Chapter 9 Novel Method for Measuring S-Nitrosothiols Using Hydrogen Sulfide",
abstract = "Recent advances in techniques that allow sensitive and specific measurement of S-nitrosothiols (RSNOs) have provided evidence for a role for these compounds in various aspects of nitric oxide (NO) biology. The most widely used approach is to couple reaction chemistry that selectively reduces RSNOs by one electron to produce NO, with the sensitive detection of the latter under anaerobic conditions using ozone based chemiluminescence in NO analyzers. Herein, we report a novel reaction that is readily adaptable for commercial NO analyzers that utilizes hydrogen sulfide (H2S), a gas that can reduce RSNO to NO and, analogous to NO, is produced by endogenous metabolism and has effects on diverse biological functions. We discuss factors that affect H2S based methods for RSNO measurement and discuss the potential of H2S as an experimental tool to measure RSNO.",
author = "Xinjun Teng and {Scott Isbell}, T. and Crawford, {Jack H.} and Bosworth, {Charles A.} and Giles, {Gregory I.} and Koenitzer, {Jeffrey R.} and Lancaster, {Jack R.} and Doeller, {Jeannette E.} and {W. Kraus}, David and {P. Patel}, Rakesh",
note = "Funding Information: This study was supported by grants from the NIH (HL71189 and HL074391 to JRL and RGM073049A to DWK) and from the American Heart Association to RPP (0655312B) and DWK (0455296B). TSI was supported by a NIH Cardiovascular Pathophysiology Training Fellowship.",
year = "2008",
doi = "10.1016/S0076-6879(08)01209-3",
language = "English",
isbn = "9780123743091",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "161--172",
booktitle = "Nitric Oxide, Part G Oxidative and Nitrosative Stress in Redox Regulation of Cell Signaling",
}