Chapter 9 Novel Method for Measuring S-Nitrosothiols Using Hydrogen Sulfide

Xinjun Teng, T. Scott Isbell, Jack H. Crawford, Charles A. Bosworth, Gregory I. Giles, Jeffrey R. Koenitzer, Jack R. Lancaster, Jeannette E. Doeller, David W. Kraus, Rakesh P. Patel

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

28 Scopus citations


Recent advances in techniques that allow sensitive and specific measurement of S-nitrosothiols (RSNOs) have provided evidence for a role for these compounds in various aspects of nitric oxide (NO) biology. The most widely used approach is to couple reaction chemistry that selectively reduces RSNOs by one electron to produce NO, with the sensitive detection of the latter under anaerobic conditions using ozone based chemiluminescence in NO analyzers. Herein, we report a novel reaction that is readily adaptable for commercial NO analyzers that utilizes hydrogen sulfide (H2S), a gas that can reduce RSNO to NO and, analogous to NO, is produced by endogenous metabolism and has effects on diverse biological functions. We discuss factors that affect H2S based methods for RSNO measurement and discuss the potential of H2S as an experimental tool to measure RSNO.

Original languageEnglish
Title of host publicationNitric Oxide, Part G Oxidative and Nitrosative Stress in Redox Regulation of Cell Signaling
PublisherAcademic Press Inc.
Number of pages12
ISBN (Print)9780123743091
StatePublished - 2008

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879


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