Changes in extracellular adenosine during chemical or electrical brain stimulation

Veronica M. Sciotti, T. S. Park, Robert M. Berne, David G.L. Van Wylen

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


The purpose of this study was to determine the changes in adenosine and adenosine metabolites during graded electrical stimulation or kainic acid-induced activation and to assess the role of adenosine in the cerebral blood flow (CBF) response to increased brain activity. A modified brain microdialysis technique was used to sample cerebral interstitial fluid (ISF), deliver drugs locally to the brain, electrically stimulate the brain, and measure local CBF (H2 clearance). Microdialysis probes were implanted bilaterally in the caudate nuclei of ketamine-anesthetized rats. Graded electrical stimulation at 5,15, and 30 Hz increased dialysate adenosine 1.5-fold, 2.3-fold, and 4.7-fold, respectively. Local infusion of kainic acid, an agonist of the excitatory amino acid neurotransmitter glutamate, produced a transient increase (2-fold) in dialysate adenosine and sustained increases in dialysate inosine (2-fold), hypoxanthine (4-fold) and CBF (2.4-fold). When the adenosine receptor antagonist 8(p-sulphophenyl)-theophylline (SPT, 10-3 M) was co-administered with kainic acid, CBF increased only 1.6-fold, while the increase in dialysate adenosine was augmented by 40%. These data demonstrate that ISF adenosine increases during brain activation and suggest that adenosine contributes to active hyperemia in the brain.

Original languageEnglish
Pages (from-to)16-20
Number of pages5
JournalBrain Research
Issue number1
StatePublished - Jun 4 1993


  • Cerebral blood flow
  • Hypoxanthine
  • Inosine
  • Kainic acid
  • Rat


Dive into the research topics of 'Changes in extracellular adenosine during chemical or electrical brain stimulation'. Together they form a unique fingerprint.

Cite this