Changes in cell surface glycoprotein expression during differentiation of human keratinocytes

C. Eberhard Klein, Carlos Cordon-Cardo, Rolf Soehnchen, Richard J. Cote, Herbert F. Oettgen, Magdalena Eisinger, Lloyd J. Old

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Six cell surface glycoproteins defined by monoclonal antibodies were selected for study on human epidermal cells. In tests on tissue sections, three of the glycoproteins [ J143 (gp 140 30); T43 (gp 85 36); H99 (gp38)] were expressed in the basal cell layer of the epidermis, whereas the other three glycoproteins [T179 (gp 140 95); T16 (gp 40 50); BT15 (gp80)] were preferentially expressed in maturing keratinocytes above the basal layer. We compared synthesis of these glycoproteins in fresh epidermis and in primary epidermal short term cultures using [35S]methionine for metabolic labeling. Synthesis of J143 was 8- to 20-fold higher and synthesis of T43 was 4- to 10-fold lower in cultured cells compared with fresh epidermis. BT15, an antigen strongly expressed on terminally differentiating keratinocytes, was synthesized at 5- to 15-fold higher levels in fresh epidermis than in cultured cells. Biosynthesis levels of H99, T179, and T16 did not change in cultured epidermal cells. Based on our findings, we propose a model of surface antigenic changes that occur during keratinocyte differentiation in vivo.

Original languageEnglish
Pages (from-to)500-506
Number of pages7
JournalJournal of Investigative Dermatology
Issue number5
StatePublished - Nov 1987


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